...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Journal of Parasitology >In vitro oncosphere-killing assays to determine immunity to the larvae of Taenia pisiformis, Taenia ovis, Taenia saginata, and Taenia solium.
【24h】

In vitro oncosphere-killing assays to determine immunity to the larvae of Taenia pisiformis, Taenia ovis, Taenia saginata, and Taenia solium.

机译：体外杀死睾丸杀死的测定，以确定Taenia Pisiformis，Taenia ovis，Taenia saginata和Taenia棒的幼虫的免疫力。

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Taeniid cestodes infect humans and livestock, causing considerable morbidity and mortality, as well as economic loss. Substantial progress has been made toward the production of recombinant vaccines against cysticercosis in livestock animals. Further development of these vaccines would be aided if a reliable in vitro test were available to measure host-protective immune responses in vaccinated animals. Here, we describe in vitro oncosphere-killing assays for the quantification of host-protective serum antibodies against Taenia pisiformis, Taenia ovis, Taenia saginata, and Taenia solium in rabbits, sheep, cattle, and pigs, respectively. Activated oncospheres of T. pisiformis, T. ovis, T. saginata, and T. solium were incubated in vitro in culture medium, test serum, and a source of complement, and oncosphere killing was assessed after 10 days of culture. In vitro oncosphere killing reflected the presence of specific antibody, and the oncosphere-killing assay typically indicated immunity to the homologous parasite that had been determined in vivo. This study describes the first reliable oncosphere-killing assays for T. pisiformis, T. ovis, T. saginata, and T. solium. These assays will be used for further research into the optimization of recombinant vaccines against cysticercosis..
机译：Taeniid Cestodes感染人类和牲畜，造成相当大的发病率和死亡率，以及经济损失。对畜禽动物中的囊尾蚴病产生重组疫苗的实质性进展。如果可靠的体外测试可用于测量接种疫苗的动物中的宿主保护免疫应答，则可以助及这些疫苗的进一步发展。在这里，我们描述了对兔塔尼亚菌，Taenia ovis，Taenia Saginata和兔子，绵羊，牛和猪的幼儿园菌，Taenia ovis，Taenia Saginata和Taenia棒的定量体外杀灭杀菌试验。在培养基，试验血清中，在体外，在培养基，试验血清和补体源中培养，在体外培养，在培养基中，在体外培养，在培养物培养后进行激活的磁力。体外跨越睾丸杀死反映了特异性抗体的存在，并且对跨越间杀灭测定通常表明对体内确定的同源寄生虫的免疫。本研究描述了第一种可靠的对肌肌，T.Ovis，T.Saginata和T.索参的杀灭基体杀灭测定。这些测定将用于进一步研究对囊霉病的重组疫苗的优化..

著录项

  • 来源
    《Journal of Parasitology》 |2006年第2期|共9页
  • 作者

    Kyngdon C T; Gauci C G; Rolfe R A; Guzman J C V; Salazar M J F; Pimentel M R V; Gonzalez A E; Garcia H H; Gilman R H; Strugnell R A; Lightowlers M W;

  • 作者单位

    Veterinary Clinical Centre The University of Melbourne 250 Princes Hwy Werribee Victoria 3030 Australia.;

    Veterinary Clinical Centre The University of Melbourne 250 Princes Hwy Werribee Victoria 3030 Australia.;

    Veterinary Clinical Centre The University of Melbourne 250 Princes Hwy Werribee Victoria 3030 Australia.;

    Veterinary Clinical Centre The University of Melbourne 250 Princes Hwy Werribee Victoria 3030 Australia.;

    Veterinary Clinical Centre The University of Melbourne 250 Princes Hwy Werribee Victoria 3030 Australia.;

    Veterinary Clinical Centre The University of Melbourne 250 Princes Hwy Werribee Victoria 3030 Australia.;

    Veterinary Clinical Centre The University of Melbourne 250 Princes Hwy Werribee Victoria 3030 Australia.;

    Veterinary Clinical Centre The University of Melbourne 250 Princes Hwy Werribee Victoria 3030 Australia.;

    Veterinary Clinical Centre The University of Melbourne 250 Princes Hwy Werribee Victoria 3030 Australia.;

    Veterinary Clinical Centre The University of Melbourne 250 Princes Hwy Werribee Victoria 3030 Australia.;

    Veterinary Clinical Centre The University of Melbourne 250 Princes Hwy Werribee Victoria 3030 Australia.;

  • 收录信息
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 寄生生物学;医学寄生虫学;
  • 关键词

    analytical methods; assays immune response; immunity in vitro; oncospheres;

    机译：分析方法;测定免疫应答;体外免疫;锚球;

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号