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首页> 外文期刊>Journal of Rubber Research >Genetic diversity analysis ofCorynespora cassiicolaisolates on the rubber tree (Hevea brasiliensis) in Vietnam using ribosomal DNA internal transcribed spacer (rDNA-ITS) sequences and sequence-related amplified polymorphism (SRAP)
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Genetic diversity analysis ofCorynespora cassiicolaisolates on the rubber tree (Hevea brasiliensis) in Vietnam using ribosomal DNA internal transcribed spacer (rDNA-ITS) sequences and sequence-related amplified polymorphism (SRAP)

机译：使用核糖体DNA内转录的间隔（RDNA-ITS）序列和序列相关扩增多态性（SRAP）越南橡胶树（HEVEA BRASILIENSIS）对越南橡胶树（HEVEA BRASILIENSIS）的遗传多样性分析

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摘要

A total of 76Corynespora cassiicolaisolates collected from 16 rubber clones in several geographic regions of Vietnam, were studied for genetic diversity by ribosomal DNA internal transcribed spacer (rDNA-ITS) sequences and sequence-related amplified polymorphism (SRAP) techniques. Results of rDNA-ITS sequence analysis indicated 2 nucleotide polymorphisms at the 135th (cytosine/thymine) and 474th (guanine/adenine) base pair positions of rDNA-ITS region, which differentiated the 76 studied isolates into 3 clusters. All isolates could be uniquely distinguished by 208 polymorphism bands which generated 93.3% polymorphic ratio with SRAP markers. A dendrogram produced from UPGMA analysis based on Nei and Li's coefficient divided the 76C. cassiicolaisolates into 2 main clusters. Cluster one included 54 fungal isolates of which 51 and 3 were observed in subgroup 1A and 1B, respectively. There were 22C. cassiicolaisolates belonging to cluster 2 with subgroups 2A and 2B consisted of 20 and 2 fungal isolates, respectively. Bootstrap values for groups one and two were 61% and 100%, respectively and the grouping was reliable, with similarity coefficient between the two main groups at 67%. rDNA-ITS sequence analysis revealed a new genetic group, signifying at least threeC. cassiicolagenetic groups that infect rubber trees in Vietnam. SRAP markers divided the studied isolates into two distinct groups, which correlated with geographical location rather than host source. To the best of our knowledge, this is the first demonstration using SRAP markers on the systematics ofC. cassiicolaisolates which cause the Corynespora leaf fall disease on various rubber (Hevea brasiliensis)clones in Vietnam.
机译：通过核糖体DNA内转录的间隔（RDNA-ITS）序列和序列相关的扩增多态性（SRAP）技术，研究了从越南地理区域收集的76corynespora孤立物从16个橡胶克隆收集的越南地理区域。 RDNA-ITS序列分析的结果表明了在第135次（胞嘧啶/胸腺嘧啶）和第474-474-474（鸟嘌呤/腺嘌呤）碱基对位置的2个核苷酸多态性，其将76研究分离为3簇。所有分离物都可以独特地区分由208多态性带，其与SRAP标记产生93.3％的多态比。基于Nei和Li的系数的UPGMA分析划分了76℃，从内部和李氏系数分析。 CASSIICOLAASOLATES成2个主要集群。在亚组1a和1b中分别观察到54个真菌分离物54个真菌分离物。有22c。与亚组2a和2b属于簇2的Cassiicolaisolates分别由20和2个真菌分离物组成。一组和二组的引导值分别为61％和100％，分组可靠，两个主要组之间的相似性系数为67％。 RDNA-其序列分析显示了一种新的遗传组，至少意味着至少Threec。感染越南橡胶树的Cassiicolagenetic组。 SRAP标记将研究的分离物分为两个不同的群体，与地理位置相关，而不是主源。据我们所知，这是使用SRAP标记对系统的第一次演示。 CASSIICOLAISOLATES引起越南各种橡胶（HEVEA BRASILIENSIS）克隆的Corynespora叶子疾病。

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