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首页> 外文期刊>Journal of separation science. >Comprehensive characterization of multiple components and metabolites of Xiaojin Capsule based on ultra high performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry
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Comprehensive characterization of multiple components and metabolites of Xiaojin Capsule based on ultra high performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry

机译：基于超高效液相色谱耦合的超高效液相色谱法综合表征Xiaojin胶囊的多元成分和代谢物与四极杆飞行时间质谱法

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摘要

Xiaojin Capsule, a classic traditional Chinese medicine formula, has been used to treat mammary cancer, thyroid nodules, and hyperplasia of the mammary glands. However, its systematic chemical information remained unclear, which hindered the interpretation of the pharmacology and the mechanism of action of this drug. In this research, an ultra high performance liquid chromatography coupled with a quadrupole time-of-flight mass spectrometry method was developed to identify the complicated components and metabolites of Xiaojin Capsule. Two acquisition modes, including the MSEnergy mode and fast data directed acquisition mode, were utilized for chemical profiling. As a result, 156 compounds were unambiguously or tentatively identified by comparing their retention times and mass spectrometry data with those of reference standards or literature. After the oral administration of Xiaojin Capsule, 53 constituents, including 24 prototype compounds and 29 metabolites, were detected in rat plasma. The obtained results were beneficial for a better understanding of the therapeutic basis of Xiaojin Capsule. A high-resolution and efficient separation method was firstly established for systematically characterizing the compounds of Xiaojin Capsule and the associated metabolites in vivo, which could be helpful for quality control and pharmacokinetic studies of this medicine.
机译：萧金胶囊是一种经典的中医配方，已被用于治疗乳腺癌，甲状腺结节和乳腺增生。然而，其系统化学信息仍然不清楚，这阻碍了药理的解释和该药物的作用机制。在该研究中，开发了一种与四极杆飞行时间质谱法相结合的超高效液相色谱法，以鉴定小金胶囊的复杂组分和代谢物。包括两个采集模式，包括MSENERGY模式和快速数据定向采集模式，用于化学分析。结果，通过将其保留时间与参考标准或文献的保留时间和质谱数据进行比较，毫不含糊地或暂时鉴定156种化合物。在大鼠等离子体中检测到Xiaojin胶囊的口服给药后，53种成分，包括24种原型化合物和29种代谢物。获得的结果有利于更好地了解小金胶囊的治疗基础。首先建立了一种高分辨率和有效的分离方法，用于系统地表征小津胶囊和体内相关代谢物的化合物，这有助于对该药的质量控制和药代动力学研究有所帮助。

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  • 来源
    《Journal of separation science.》 |2019年第17期|共14页
  • 作者

    Qi Xiaopo; Wang Xinyu; Cheng Taofang; Wu Qiuling; Mi Nan; Mu Xuemei; Guo Xin; Zhao Gang; Huang Zhijun; Ye Ji; Zhang Weidong;

  • 作者单位

    Fujian Univ Tradit Chinese Med Dept Pharm Fuzhou 350122 Fujian Peoples R China;

    Anhui Univ Chinese Med Sch Pharm Hefei Anhui Peoples R China;

    Shanghai Jiao Tong Univ Sch Pharm Shanghai Peoples R China;

    Fujian Univ Tradit Chinese Med Dept Pharm Fuzhou 350122 Fujian Peoples R China;

    Second Mil Med Univ Sch Pharm Dept Phytochem Shanghai Peoples R China;

    Second Mil Med Univ Sch Pharm Dept Phytochem Shanghai Peoples R China;

    Second Mil Med Univ Sch Pharm Dept Phytochem Shanghai Peoples R China;

    Jianmin Pharmaceut Grp R&

    D Ctr Wuhan Hubei Peoples R China;

    Jianmin Pharmaceut Grp R&

    D Ctr Wuhan Hubei Peoples R China;

    Second Mil Med Univ Sch Pharm Dept Phytochem Shanghai Peoples R China;

    Fujian Univ Tradit Chinese Med Dept Pharm Fuzhou 350122 Fujian Peoples R China;

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  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 化学;
  • 关键词

    chemical components; mass spectrometry; metabolites; traditional Chinese medicine;

    机译：化学成分;质谱;代谢物;中药;

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