Comprehensive characterization and quantification of multiple components in Dan‐Huang‐Qu‐Yu capsule using a multivariate data processing approach based on microwave‐assisted extraction with UHPLC and Q Exactive quadrupole‐orbitrap high‐resolution mass spectrometry

Yang Bo; Ying Yin; Zou Jie; Ge Shuyu; Zuo Lihua

摘要

Abstract > Dan‐Huang‐Qu‐Yu capsule, a Chinese herbal medicine compound preparation, is widely used for chronic pelvic inflammatory disease. In this study, a rapid, selective, and sensitive microwave‐assisted extraction ultra‐high‐performance liquid chromatography‐Q Exactive quadrupole‐orbitrap high‐resolution mass spectrometry method was developed for analyzing its chemical compositions. A total of 85 compounds, including 22 flavonoids, 8 terpenoids, 5 quinones, 5 phthaleolactone, 23 organic acids, and 22 other compounds were identified from Dan‐Huang‐Qu‐Yu capsule. Among them, 35 major compounds were unambiguously detected by comparing them with reference standards and selected as quality control markers, which were simultaneously determined in Dan‐Huang‐Qu‐Yu capsule. The established method was successfully validated and applied for simultaneous determination of 35 bioactive compounds in Dan‐Huang‐Qu‐Yu capsule from ten sample batches. The quantitative data of the analytes were analyzed by principal component analysis for quality assessment of Dan‐Huang‐Qu‐Yu capsule. Six compounds (e.g., astragaloside IV, salvianolic acid B, ellagic acid, chlorogenic acid, N ‐butylidenephthalide, and luteolin) were screened out and regarded as chemical markers for quality control of Dan‐Huang‐Qu‐Yu capsule. The established method has been proved to be a novel and useful tool for rapid research of Dan‐Huang‐Qu‐Yu capsule. This research will provide reference for the scientific research of traditional Chinese medicines. </abstract> </span> <span class="z_kbtn z_kbtnclass hoverxs" style="display: none;">展开▼</span> </div> <div class="translation abstracttxt"> <span class="zhankaihshouqi fivelineshidden" id="abstract"> <span>机译：</span><abstract type =“main”xml：lang =“en”> <title type =“main”>抽象</ title> > Dan-huang-qu-yu胶囊，中草药复合制剂，被广泛使用用于慢性盆腔炎炎症疾病。在该研究中，开发了一种快速，选择性和敏感的微波辅助提取超高性能液相色谱 - Q辐射高度高度液相色谱法，用于分析其化学组成。总共85种化合物，包括22种黄酮，8萜类化合物，5次醌，5邻黄曲玉胶囊鉴定出22种其它化合物。其中，通过将35种主要化合物与参考标准进行比较并选择为质量控制标志物，其在丹 - 黄曲玉胶囊中同时测定。成功验证了已建立的方法，并应用于来自十个样品批次的丹 - 黄曲囊胶囊中35种生物活性化合物。分析物的定量数据是通过丹 - 黄曲岩胶囊质量评估的主要成分分析分析。筛选出筛选六种化合物（例如，黄芪，Salvianolic酸B，鞣酸，氟酸，丁二酸， - 丁苯妥苯胺和虎蛋白），并被视为丹 - 黄曲的质量控制的化学标志物 - 俞胶囊。已被证明是丹黄曲岩胶囊快速研究的新颖且有用的工具。该研究将为中药科研提供参考。 </ p> </ abstract> </span> <span class="z_kbtn z_kbtnclass hoverxs" style="display: none;">展开▼</span> </div> </div> <div class="record"> <h2 class="all_title" id="enpatent33" >著录项</h2> <ul> <li> <span class="lefttit">来源</span> <div style="width: 86%;vertical-align: text-top;display: inline-block;"> <a href='/journal-foreign-35800/'>《Journal of separation science.》</a> <b style="margin: 0 2px;">|</b><span>2019年第11期</span><b style="margin: 0 2px;">|</b><span>共11页</span> </div> </li> <li> <div class="author"> <span class="lefttit">作者</span> <p id="fAuthorthree" class="threelineshidden zhankaihshouqi"> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Yang Bo&option=202" target="_blank" rel="nofollow">Yang Bo;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Ying Yin&option=202" target="_blank" rel="nofollow">Ying Yin;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Zou Jie&option=202" target="_blank" rel="nofollow">Zou Jie;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Ge Shuyu&option=202" target="_blank" rel="nofollow">Ge Shuyu;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Zuo Lihua&option=202" target="_blank" rel="nofollow">Zuo Lihua;</a> </p> <span class="z_kbtnclass z_kbtnclassall hoverxs" id="zkzz" style="display: none;">展开▼</span> </div> </li> <li> <div style="display: flex;"> <span class="lefttit">作者单位</span> <div style="position: relative;margin-left: 3px;max-width: 639px;"> <div class="threelineshidden zhankaihshouqi" id="fOrgthree"> <p>Department of PharmacyTongde Hospital Zhejiang ProvinceHangzhou Zhejiang P. R. China;</p> <p>Department of PharmacyTongde Hospital Zhejiang ProvinceHangzhou Zhejiang P. R. China;</p> <p>Department of Pharmacythe 117th Hospital of PLAHangzhou Zhejiang P. R. China;</p> <p>Department of PharmacyTongde Hospital Zhejiang ProvinceHangzhou Zhejiang P. R. China;</p> <p>Department of PharmacyThe First Affiliated Hospital of Zhengzhou UniversityZhengzhou Henan Province P. R. China;</p> </div> <span class="z_kbtnclass z_kbtnclassall hoverxs" id="zhdw" style="display: none;">展开▼</span> </div> </div> </li> <li > <span class="lefttit">收录信息</span> <span style="width: 86%;vertical-align: text-top;display: inline-block;"></span> </li> <li> <span class="lefttit">原文格式</span> <span>PDF</span> </li> <li> <span class="lefttit">正文语种</span> <span>eng</span> </li> <li> <span class="lefttit">中图分类</span> <span><a href="https://www.zhangqiaokeyan.com/clc/159.html" title="化学">化学;</a></span> </li> <li class="antistop"> <span class="lefttit">关键词</span> <p style="width: 86%;vertical-align: text-top;"> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=high‐resolution mass spectrometry&option=203" rel="nofollow">high‐resolution mass spectrometry;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=microwave‐assisted extraction&option=203" rel="nofollow">microwave‐assisted extraction;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=principal component analysis&option=203" rel="nofollow">principal component analysis;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=quality assessment&option=203" rel="nofollow">quality assessment;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=traditional Chinese medicine&option=203" rel="nofollow">traditional Chinese medicine;</a> </p> <div class="translation"> 机译：高分辨率质谱;微波辅助提取;主成分分析;质量评估;中医; </div> </li> </ul> </div> </div> <div class="literature cardcommon"> <div class="similarity "> <h3 class="all_title" id="enpatent66">相似文献</h3> <div class="similaritytab clearfix"> <ul> <li class="active" >外文文献</li> </ul> </div> <div class="similarity_details"> <ul > <li> <div> <b>1. </b><a class="enjiyixqcontent" href="/journal-foreign-detail/0704025846556.html">Comprehensive characterization and quantification of multiple components in 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