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首页> 外文期刊>American journal of dentistry >Microtensile bond strength of etch-and-rinse and self-etch adhesive systems to demineralized dentin after the use of a papain-based chemomechanical method.
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Microtensile bond strength of etch-and-rinse and self-etch adhesive systems to demineralized dentin after the use of a papain-based chemomechanical method.

机译：使用基于木瓜蛋白酶的化学机械方法后，蚀刻和冲洗以及自蚀刻粘合剂系统对脱矿质牙本质的微拉伸强度。

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PURPOSE: To evaluate the in vitro microtensile bond strength (microTBS) of etch-and-rinse and self-etch adhesive systems to demineralized dentin after the use of a papain-based chemomechanical method. METHODS: 36 demineralized human dentin slabs were randomly distributed into two groups according to the method of caries removal: (1). Mechanical removal with manual excavators; (2) Chemomechanical removal with a papain-based gel (Papacarie). Subsequently, three adhesive systems were applied (n=6): (a) an etch-and-rinse adhesive system (Single Bond); (b) a two-step self-etch adhesive system (AdheSE); (c) a one-step self-etch adhesive system (Adper Prompt). The slabs were restored with a microhybrid resin composite and each resin-dentin block was sectioned into 1.0 mm2 thick slabs, which were kept in receptacles containing distilled water at relative humidity, for 24 hours, at 37 degrees C. After that, they were subjected to tensile stress in a universal testing machine at a speed of 0.5 mm/minute. Data were submitted to two-way ANOVA and Tukey's test at a 0.05 level of significance. The fractured specimens were observed under a stereomicroscope to assess the failure mode. RESULTS: The application of both chemomechanical and mechanical methods on demineralized dentin yielded microTBS values that were statistically similar among them, regardless of the adhesive system used. Caries removal with a chemomechanical papain-based method did not interfere in the adhesion of the tested adhesive systems to demineralized dentin.
机译：目的：在使用基于木瓜蛋白酶的化学机械方法后，评估去离子和去矿质牙本质的蚀刻和冲洗和自蚀刻粘合剂系统的体外微拉伸粘合强度（microTBS）。方法：根据龋齿去除方法将36块脱矿质人牙本质板随机分为两组：（1）。用手动挖掘机进行机械拆除; （2）用木瓜蛋白酶凝胶（Papacarie）进行化学机械去除。随后，应用了三种粘合系统（n = 6）：（a）蚀刻和冲洗粘合系统（单键）; （b）两步自蚀刻粘合剂系统（AdheSE）; （c）一步式自蚀刻粘合剂系统（Adper Prompt）。用微混合树脂复合材料修复这些平板，并将每个树脂-牙本质块切成1.0 mm2厚的平板，将其在37°C下于相对湿度的装有蒸馏水的容器中放置24小时。之后，对其进行处理。在万能试验机中以0.5毫米/分钟的速度拉伸应力。数据以0.05的显着性水平提交至双向ANOVA和Tukey检验。在立体显微镜下观察断裂的样品，以评估破坏模式。结果：化学机械方法和机械方法在脱矿牙本质上的应用均产生了microTBS值，无论使用何种粘合剂系统，它们之间的统计学差异均相似。使用基于化学机械木瓜蛋白酶的方法去除龋齿不会干扰所测试的粘合剂系统与脱矿牙本质的粘附。

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