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首页> 外文期刊>Journal of stroke and cerebrovascular diseases: The official journal of National Stroke Association >Neuroprotective Effect of Dichloromethane Extraction From Piper nigrum L. and Piper longum L. on Permanent Focal Cerebral Ischemia Injury in Rats
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Neuroprotective Effect of Dichloromethane Extraction From Piper nigrum L. and Piper longum L. on Permanent Focal Cerebral Ischemia Injury in Rats

机译：二氯甲烷萃取从吹笛剂NIGRUM L.和PIPER Longum L.对大鼠永久性局灶性脑缺血损伤的神经保护作用

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摘要

Background: Piper nigrum L. and Piper longum L. consist a classic formula in traditional Chinese Hui medicine and are widely used in treatment of stroke. To examine the therapeutic effect of neuron injury after apoplexy, we used a permanent middle cerebral artery occlusion model in rats to investigate the effects of dichloromethane fraction (DF) of Piper nigrum L. and Piper longum L. Materials and Methods: After subjecting the rats to permanent middle cerebral artery occlusion, DF (100 and 200 mg/kg) were administered for 14 days. Neurological deficits and the degree of cerebral tissue injury was detected by 2,3,5-Triphenyltetrazolium Chloride Staining Hematoxylin and eosin staining and Nissl staining. Postsynaptic density protein 95 (PSD-95), synapsin-I (syn-I), and a.-synuclein (a.-syn) were stained by immunohistochemistry. PSD-95, Ca2+ /calmodulin (CaM)-dependent protein kinase II (CaMK II), phosphorylated CaMK II (p-CaMK II), CaM, N-methyl D-aspartate receptor subtype 2B (NR2B) expression were detected by Western blot. Meanwhile, phytochemical profile of DF was determined through ultrahigh-performance liquid chromatography-quadrupole time-of-flight mass spectrometry (UPLC-Q-TOF/MS). Results: DF alleviated neurological deficits and markedly prevented ischemia-induced cellular damage. Immunohistochemical micrographs revealed that PSD-95 and syn-I proteins increased, and alpha-syn presented reduced expression in brain samples from the sham group. Western blot analyses revealed that the model group exhibited a noticeable reduction in PSD-95, p-CaMK II, CaM, and NR2B. The DF-treated model group exhibited increased PSD-95, p-CaMK II, CaM, and NR2B. UPLC-Q-TOF/MS analysis revealed eight main components of DF, of which piperine accounted for the largest proportion.
机译：背景：吹笛者Nigrum L.和Piper Longum L.在中国中辉中的一种经典配方组成，广泛用于治疗中风。为了检查中风后神经元损伤的治疗效果，我们在大鼠中使用永久性中脑动脉闭塞模型，研究了吹笛剂Nigrum L.和Piper Longum L.材料和方法的二氯甲烷级分（DF）的影响：对大鼠进行大鼠后对于永久性中脑动脉闭塞，将DF（100和200mg / kg）施用14天。通过2,3,5-三苯基四氯铵染色苏木精和曙红染色和肉桂染色来检测神经系统缺陷和脑组织损伤程度。通过免疫组织化学染色突触后密度蛋白质95（PSD-95），Synapsin-I（Syn-I）和A.-突触核蛋白（A.-SYN）。 PSD-95，CA2 + /钙调蛋白（CAM） - 依赖性蛋白激酶II（CAMK II），磷酸化CAMK II（P-CAMK II），CAM，N-甲基D-天冬氨酸受体亚型2B（NR2B）表达被Western印迹检测到。同时，通过超高性能液相色谱 - 四极针对飞行时间质谱法（UPLC-Q-TOF / MS）确定DF的植物化学分布。结果：DF缓解神经系统缺陷并显着防止缺血诱导的细胞损伤。免疫组织化学显微照片显示PSD-95和Syn-I蛋白增加，α-SYN呈现来自假手术组的脑样品中的表达。 Western Blot分析显示，模型组在PSD-95，P-Camk II，Cam和NR2B中表现出明显的减少。 DF处理的模型组显示PSD-95，P-Camk II，CAM和NR2B增加。 UPLC-Q-TOF / MS分析显示DF的八个主要成分，其中哌啶占比例最大。

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