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首页> 外文期刊>Journal of stroke and cerebrovascular diseases: The official journal of National Stroke Association >Liraglutide Protects Neurite Outgrowth of Cortical Neurons Under Oxidative Stress though Activating the Wnt Pathway
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Liraglutide Protects Neurite Outgrowth of Cortical Neurons Under Oxidative Stress though Activating the Wnt Pathway

机译：Liraglutide在氧化应激下保护皮质神经元的神经沸虫过多，尽管激活Wnt途径

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BackgroundNeurogenesis including neurite outgrowth is important for brain plasticity under physiological conditions and in brain repair after injury. Liraglutide has been found to have neuroprotective action in the risk of central nervous system disease. However, the effect and the potential mechanism of liraglutide-induced neurite outgrowth in primary cortical neurons under oxidative stress remain poorly documented. MethodsIn the text, H2O2was used to mimic ischemia injury in primary cortical neurons. The viability and apoptosis of cell was assessed by Cell Counting Kit-8 and Hoechst 33342. Immunofluorescence method was used to examine the effect of liraglutide on neurite outgrowth in cortical neuron under H2O2condition. Then, the potential mechanisms involving the Wnt pathway were investigated. The expression of β-catenin, c-myc, and cyclin D1 was determined using quantitative real-time polymerase chain reaction and Western blot. ResultsLiraglutide significantly increased the viability and alleviated the apoptosis rate of cortical neurons induced by H2O2. Next, liraglutide promoted neurite outgrowth, which could be partially inhibited by the Wnt pathway inhibitor Xav939. Besides, liraglutide induced an increase of β-catenin, c-myc, and cyclin D1 levels, which could also be blocked in the presence of Xav939. ConclusionsThese results illustrate that liraglutide exerts neurotrophin-like activity in cortical neurons under oxidative stress condition, partly through activating the Wnt pathway.
机译：包括神经沸肌产物的背景对生理条件下的脑塑性和伤害后脑修复都很重要。已发现丽格勒胺对中枢神经系统疾病的风险具有神经保护作用。然而，在氧化应激下初级皮质神经元在原发性皮质神经元中的兴奋性诱导的神经突生长的效果和潜在机制仍然有难以记录。方法，文本，H2O2Was用于模拟原发性皮质神经元的缺血损伤。通过细胞计数试剂盒-8和Hoechst 33342评估细胞的可行性和凋亡。使用H2O 2 Codition探测Liraglutide对Liraglutide对H2O 2 Co.cn下皮质神经元中神经沸肌产物的影响。然后，研究了涉及WNT途径的潜在机制。使用定量的实时聚合酶链反应和Western印迹测定β-连环蛋白，C-myc和Cyclin D1的表达。结果罗拉格蛋白质显着提高了活力并减轻了H2O2诱导的皮质神经元的凋亡率。接下来，Liraglutide促进了神经突的过度，可以由Wnt途径抑制剂XAV939部分抑制。此外，Liraglutide诱导β-连环蛋白，C-MYC和细胞周期蛋白D1水平的增加，这也可以在XAV939的存在下封闭。结论结果表明，在氧化胁迫条件下，丽菌蛋白酶在皮质神经元中施加神经滋生素样活性，部分通过激活WNT途径。

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