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首页> 外文期刊>Biochimica et biophysica acta: BBA: International journal of biochemistry, biophysics and molecular biololgy. Proteins and Proteomics >hIscA: a protein implicated in the biogenesis of iron-sulfur clusters
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hIscA: a protein implicated in the biogenesis of iron-sulfur clusters

机译：hIscA：一种蛋白质，与铁硫簇的生物发生有关

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This article describes the gene called hIscA, its transcription product and protein (hIscA) and its putative function. We screened a human brain cDNA expression library with serum from a patient suffering from the autoimmune Sj?gren's syndrome (S5:823/94). One cDNA of 1.6-kbp clone was isolated. This clone contains the entire coding sequence for a protein unknown in human. IscA is ubiquitously expressed and expression levels vary among tissues. The 15.5-kDa predicted protein contains a structural domain named HESB, is located in the mitochondria and is implicated in the biogenesis of iron–sulfur clusters. Since this unknown protein is related to IscA-like protein, we suggest as name for this protein hIscA. The recombinant protein is recognized by a rabbit polyclonal antiserum generated against the carboxyl extreme of the Saccharomyces cerevisiae orthologue Isa1. In this article, we demonstrate the functional homology between hIscA and Isa1 proteins using Isa1 null mutant S. cerevisiae transformed with hIscA in a yeast functional complementation test. We also describe the rat homologue to this gene.
机译：本文介绍了称为hIscA的基因，其转录产物和蛋白质（hIscA）及其推定功能。我们用患有自身免疫性干燥综合征的患者的血清筛选了人脑cDNA表达文库（S5：823/94）。分离出一个1.6kbp克隆的cDNA。该克隆包含人类未知蛋白的完整编码序列。 IscA被普遍表达，并且表达水平在组织之间变化。 15.5kDa预测蛋白包含一个名为HESB的结构域，位于线粒体中，并与铁硫簇的生物发生有关。由于该未知蛋白与IscA样蛋白有关，因此建议将其命名为hIscA。重组蛋白被针对啤酒酵母直系同源物Isa1的羧基末端的兔多克隆抗血清所识别。在本文中，我们在酵母功能互补测试中展示了使用hIscA转化的Isa1空突变酿酒酵母对hIscA和Isa1蛋白之间的功能同源性。我们还描述了该基因的大鼠同源物。

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