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首页> 外文期刊>Journal of the mechanical behavior of biomedical materials >Fabrication, mechanical property and in vitro evaluation of poly (L-lactic acid-co-epsilon-caprolactone) core-shell nanofiber scaffold for tissue engineering
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Fabrication, mechanical property and in vitro evaluation of poly (L-lactic acid-co-epsilon-caprolactone) core-shell nanofiber scaffold for tissue engineering

机译：聚（L-乳酸 - 共ε-己内酮）核 - 壳纳米纤维支架用于组织工程的制备，力学性能和体外评价

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Coaxial electrospinning, in which Poly (L-lactic acid-co-epsilon-caprolactone) (PLC) with different Lactic acid (LA) to caprolactone (CL) ratio (75:25 and 50:50) were employed to electrospin core-shell nanofibers which could mimic the native extracellular matrix for tissue engineering applications. Core-shell nanofibrous scaffolds of PLC (50:50)/BSA (426 +/- 157 nm) and PLC (75:25)/BSA (427 +/- 197 nm) were fabricated and model drug bovine serum albumin (BSA) was entrapped in the core layer. The morphology, core-shell structure and sustained release behaviors were evaluated by Scanning electron microscopy (SEM), transmission electron microscopy (TEM), inverted fluorescence microscopy, water contact angle test and in vitro release test, respectively. The effect of core-shell structure and shell layer materials on the variation tendency of mechanical characterization in dry and wet situation were also investigated by tensile testing. The in vitro biocompatibility of scaffolds were investigated by growing human mesenchymal stem cells (hMSCs) on scaffolds surface and the proliferation of cells were evaluated with Alamar Blue tests. In vitro cultivations of hMSCs showed that PLC (50:50)/BSA scaffolds supported a significantly higher proliferation rate of seeded cells than scaffolds prepared by polymer PLC (75:25)/BSA. Overall, the PLC core-shell nanofibers possessed potentially regulable mechanical properties useful for tissue engineering as well as sustained release potential for medical applications.
机译：同轴静电纺丝，其中将具有不同乳酸（La）与己内酯（Cl）比（75:25和50:50）的聚（L-乳酸 - Co-epsilon-caprolacterone）（PLC）用于Electrose素核心 - 壳纳米纤维可以模拟动物工程应用的天然细胞外基质。 PLC核心壳纳米纤维支架（50:50）/ BSA（426 +/- 157nm）和PLC（75:25）/ BSA（427 +/- 197nm）是制造的，并模拟药物牛血清白蛋白（BSA）被夹在核心层。通过扫描电子显微镜（SEM），透射电子显微镜（TEM），倒荧光显微镜，水接触角测试和体外释放试验，评估了形态，核心壳结构和持续释放行为。通过拉伸试验还研究了核 - 壳结构和壳层材料对干燥和湿地区的机械表征变化趋势的影响。通过在支架表面上生长人间充质干细胞（HMSC）来研究支架的体外生物相容性，并用Alamar蓝试验评估细胞的增殖。 HMSC的体外培养表明，PLC（50:50）/ BSA支架支持比聚合物PLC（75:25）/ BSA制备的支架的种子细胞的显着较高的加热速率。总体而言，PLC核心壳纳米纤维具有可用于组织工程的可能可调节的机械性能，以及用于医疗应用的持续释放潜力。

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