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首页> 外文期刊>Journal of the Indian Chemical Society >Induction of mutation and development of a high L-tryptophan yielding Bacillus subtilis
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Induction of mutation and development of a high L-tryptophan yielding Bacillus subtilis

机译:高L-色氨酸的突变和发育诱导芽孢杆菌枯草芽孢杆菌

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摘要

A regulatory mutant Bacillus subtilis GSX1159 was employed for the development of high-tryptophan yielding strain via random mutation using ethylelemine and UV irradiations. It followed selection and protoplast fusion between high L-threonine yielding strain and multiple L-threonine analog-resistant strain to develop such a high yielding strain which can prevent feedback inhibition. A high L-threonine yielding strain Bacillus subtilis GSX1794 was developed by induced mutation. It was then subjected to protoplast fusion with multiple L-threonine analog-resistant strain Bacillus subtilis GSX2170. It led to the development of a multiple analog-resistant high L-threonine yielding strain Bacillus subtilis GSX2480, which could accumulate up to 8.3 mg/ml L-threonine (64.3% greater accumulation than Bacillus subtilis GSX1159) in the fermentation broth on subsequent fermentation trials.
机译:使用乙基脲素和紫外线照射,使用调节突变体枯草芽孢杆菌GSX1159通过随机突变通过随机突变进行高蛋白突变。 接下来是高L-苏氨酸产生应变和多种L-苏氨酸模拟耐菌株之间的选择和原生质体融合,以开发这种高产菌株,可以防止反馈抑制。 通过诱导突变开发了一种高L-苏氨酸菌株枯草芽孢杆菌GSX1794。 然后将其与多种L-苏氨酸类似物抗性菌株Bacillus枯草芽孢杆菌GSX2170进行原生质体融合。 它导致了多种模拟抗性高L-苏氨酸产生菌株芽孢杆菌GSX2480,其在后续发酵的发酵液中,可以积聚高达8.3mg / ml的L-苏氨酸(比枯草芽孢杆菌GSX1159比枯草芽孢杆菌GSX1159更大的累积64.3%) 试验。

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