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首页> 外文期刊>American journal of botany >Identification and characterization of 43 novel polymorphic EST-SSR markers for arum lily, Zantedeschia aethiopica (Araceae).
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Identification and characterization of 43 novel polymorphic EST-SSR markers for arum lily, Zantedeschia aethiopica (Araceae).

机译:鉴定和表征43种新颖的多态EST-SSR标记,用于百合百合,拟南芥(Araceae)。

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摘要

Premise of the study: A new set of microsatellite or simple sequence repeat (SSR) markers from expressed sequence tags (ESTs) was developed for arum lily (Zantedeschia aethiopica), which is one of the most iconic and widely recognized ornamental plants in the world. Methods and Results: Using 2175 unigenes derived from 4283 random ESTs in arum lily, 166 primer pairs were designed and tested for amplification in 24 accessions from Asia, Europe, and Africa. A total of 43 loci were polymorphic, with the number of alleles per locus ranging from two to 10. The observed heterozygosity, expected heterozygosity, and polymorphism information content ranged from 0.2313 to 0.8480, 0.3034 to 0.8648, and 0.1015 to 0.7364, respectively. Conclusions: These novel polymorphic EST-SSR markers will facilitate future studies of genetic variation and molecular-assisted breeding systems in arum lily.Digital Object Identifier http://dx.doi.org/10.3732/ajb.1200228
机译:研究的前提:针对百合(Zantedeschia aethiopica)开发了一套新的微卫星或表达序列标签(EST)的简单序列重复(SSR)标记,该百合是世界上最具标志性和最广为人知的观赏植物之一。方法和结果:使用来自香椿属4283个随机EST的2175个单基因,设计并测试了166个引物对,用于在来自亚洲,欧洲和非洲的24个种质中进行扩增。共有43个基因座是多态性的,每个位点的等位基因数量在2到10之间。观察到的杂合性,预期杂合性和多态性信息含量分别为0.2313至0.8480、0.3034至0.8648和0.1015至0.7364。结论:这些新颖的多态EST-SSR标记将有助于今后对金莲花的遗传变异和分子辅助育种系统的研究。数字对象标识符http://dx.doi.org/10.3732/ajb.1200228

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