...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Journal of tissue engineering and regenerative medicine >RGD‐functionalized polyurethane scaffolds promote umbilical cord blood mesenchymal stem cell expansion and osteogenic differentiation
【24h】

RGD‐functionalized polyurethane scaffolds promote umbilical cord blood mesenchymal stem cell expansion and osteogenic differentiation

机译：RGD-官能化聚氨酯支架促进脐带血间充质干细胞膨胀和成骨分化

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Abstract Biomimetic materials are essential for the production of clinically relevant bone grafts for bone tissue engineering applications. Their ability to modulate stem cell proliferation and differentiation can be used to harness the regenerative potential of those cells and optimize the efficiency of engineered bone grafts. The arginyl‐glycyl‐aspartic acid (RGD) peptide has been recognized as the adhesion motif of various extracellular matrix proteins and can affect stem cell behaviour in biomaterials. Attempts to functionalize biomaterials with RGD have been limited to a maximum of 1‐ to 3‐mm thickness scaffolds, overlooking the issue of core infiltration that represents a major hurdle in developing real thickness scaffolds. Herein, we present the cross‐linking of RGD on the surface of “real thickness” (5?×?5?×?5?mm) porous polyurethane scaffolds (PU‐RGD), to be used for the expansion and osteogenic differentiation of umbilical cord blood mesenchymal stem cells (UCB MSCs). RGD‐functionalized scaffolds increased initial cell adhesion (1.5‐fold to twofold) and achieved a 3.4‐fold increase in cell numbers at the end of culture compared with a 1.5‐fold increase in non‐functionalized controls. Homogenous cell infiltration to the scaffold core was observed in the PU‐RGD scaffolds. Importantly, PU‐RGD scaffolds were able to enhance the osteogenic differentiation of UCB MSCs. Osteogenic gene and protein expression increased in scaffolds functionalized with 100?μg/ml RGD. Higher RGD concentrations (200?μg/ml) were less efficient in stimulating osteogenic differentiation. We conclude that robust RGD tethering to 3D PU “real thickness” scaffolds is possible and that it promotes core infiltration, expansion, and osteogenic differentiation of UCB MSCs for the purposes of bone regeneration.
机译：摘要仿生材料对于生产临床相关的骨移植物是必不可少的骨组织工程应用。它们调节干细胞增殖和分化的能力可用于利用这些细胞的再生潜力，并优化工程骨移植的效率。已识别氨基 - 甘氨酸 - 天冬氨酸（RGD）肽作为各种细胞外基质蛋白的粘附基质，并且可以影响生物材料中的干细胞行为。尝试用RGD挥发生物材料的厚度尺寸尺寸为1至3毫米，俯瞰核心渗透问题，该问题代表了开发真实厚度支架的主要障碍。在此，我们介绍了RGD在“真实厚度”（5？×5？5？5？5？5？5？5Ω·×5×5？5Ω·×5×5×5？5×5×5？5×5×5×5·×5·×5×5·×5×5·×5·×5·×5×5·×5×5·×5·×5×5·×5·×5·×5·×5·×5·×5·×5·×5·×5×5·×5·×5·×5·×5·×5·×5·×5·×5·×5·×5·×5？脐带血间充质干细胞（UCB MSCs）。 RGD-官能化支架增加初始细胞粘附（1.5倍至双倍），并在培养物结束时达到3.4倍的细胞数增加，而非官能化对照的1.5倍增加。在PU-RGD支架中观察到支架核心的均匀细胞浸润。重要的是，PU-RGD支架能够增强UCB MSCs的成骨分化。成骨基因和蛋白质表达在用100μg/ ml RGD官能化的支架中增加。在刺激成骨分化中，较高的RGD浓度（200μgμg/ ml）较低。我们得出结论，稳健的RGD束缚到3D PU“实厚度”支架是可能的，并且它促进了UCB MSCS的核心浸润，膨胀和成骨分化，以便骨再生的目的。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号