...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Journal of tissue engineering and regenerative medicine >Hypoxic preconditioning of myoblasts implanted in a tissue engineering chamber significantly increases local angiogenesis via upregulation of myoblast vascular endothelial growth factor-A expression and downregulation of miRNA-1, miRNA-206 and angiopoietin-1
【24h】

Hypoxic preconditioning of myoblasts implanted in a tissue engineering chamber significantly increases local angiogenesis via upregulation of myoblast vascular endothelial growth factor-A expression and downregulation of miRNA-1, miRNA-206 and angiopoietin-1

机译：植入组织工程室中的肌细胞的缺氧预处理通过Ucoblast血管内皮生长因子的上调显着提高了局部血管生成 - miRNA-1，miRNA-206和血管发球子-1的表达和下调

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Vascularization is a major hurdle for growing three-dimensional tissue engineered constructs. This study investigated the mechanisms involved in hypoxic preconditioning of primary rat myoblasts in vitro and their influence on local angiogenesis postimplantation. Primary rat myoblast cultures were exposed to 90min hypoxia at 1% oxygen followed by normoxia for 24h. Real time (RT) polymerase chain reaction evaluation indicated that 90min hypoxia resulted in significant downregulation of miR-1 and miR-206 (p 0.05) and angiopoietin-1 (p 0.05) with upregulation of vascular endothelial growth factor-A (VEGF-A; p 0.05). The miR-1 and angiopoietin-1 responses remained significantly downregulated after a 24h rest phase. In addition, direct inhibition of miR-206 in L6 myoblasts caused a significant increase in VEGF-A expression (p 0.05), further establishing that changes in VEGF-A expression are influenced by miR-206. Of the myogenic genes examined, MyoD was significantly upregulated, only after 24h rest (p 0.05). Preconditioned or control myoblasts were implanted with Matrigel into isolated bilateral tissue engineering chambers incorporating a flow-through epigastric vascular pedicle in severe combined immunodeficiency mice and the chamber tissue harvested 14days later. Chambers implanted with preconditioned myoblasts had a significantly increased percentage volume of blood vessels (p = 0.0325) compared with chambers implanted with control myoblasts. Hypoxic preconditioned myoblasts promote vascularization of constructs via VEGF upregulation and downregulation of angiopoietin-1, miR-1 and miR-206. The relatively simple strategy of hypoxic preconditioning of implanted cells - including non-stem cell types - has broad, future applications in tissue engineering of skeletal muscle and other tissues, as a technique to significantly increase implant site angiogenesis.
机译：血管化是一种越来越多的三维组织工程构建体的主要障碍。本研究调查了体外对原发药肌细胞的缺氧预处理的机制及其对局部血管生成后的影响。原发性大鼠肌细胞培养物在＆lt中暴露于90min缺氧。 1％氧气，然后是常氧氧化24小时。实时（RT）聚合酶链反应评价表明，90min缺氧导致MIR-1和MIR-206（P <0.05）的显着下调（P <0.05），血管内皮生长因子-A的上调是血管内皮生长因子-A的上调（VEGF-A; P <0.05）。在24h静态阶段后，miR-1和血管血红素-1反应仍然明显下调。此外，L6肌细胞中的miR-206的直接抑制导致VEGF-A表达的显着增加（P <0.05），进一步建立VEGF-A表达的变化受MIR-206的影响。在检查的肌遗传学基因中，仅在24小时后显着上调MyOD（P <0.05）。将预处理或对照肌细胞植入Mattigel中，进入含有严重组合免疫缺陷小鼠中的流通血管血管椎弓集的分离的双侧组织工程室，并且腔室组织以后收获14天。与植入对照肌细胞的腔室相比，植入预处理肌细胞的血管百分比体积显着增加（P = 0.0325）。缺氧预处理肌细胞通过VEGF上调促进构建体的血管化，下调血管发成素-1，miR-1和miR-206。植入细胞缺氧预处理的相对简单的策略 - 包括非干细胞类型 - 具有广泛的未来应用在骨骼肌和其他组织组织工程中，作为显着增加植入物位点血管生成的技术。

著录项

相似文献

  • 外文文献
  • 中文文献
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号