...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Journal of tissue engineering and regenerative medicine >Exogenous transforming growth factor-beta 1 enhances smooth muscle differentiation in embryonic mouse jejunal explants
【24h】

Exogenous transforming growth factor-beta 1 enhances smooth muscle differentiation in embryonic mouse jejunal explants

机译：外源性转化生长因子-β1增强了胚胎小鼠赤腔牛外植体的平滑肌分化

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

An ex vivo experimental strategy that replicates in vivo intestinal development would in theory provide an accessible setting with which to study normal and dysmorphic gut biology. The current authors recently described a system in which mouse embryonic jejunal segments were explanted onto semipermeable platforms and fed with chemically defined serum-free media. Over 3 days in organ culture, explants formed villi and they began to undergo spontaneous peristalsis. As defined in the current study, the wall of the explanted gut failed to form a robust longitudinal smooth muscle (SM) layer as it would do in vivo over the same time period. Given the role of transforming growth factor beta 1 (TGF beta 1) in SM differentiation in other organs, it was hypothesized that exogenous TGF beta 1 would enhance SM differentiation in these explants. In vivo, TGF beta receptors I and II were both detected in embryonic longitudinal jejunal SM cells and, in organ culture, exogenous TGF beta 1 induced robust differentiation of longitudinal SM. Microarray profiling showed that TGF beta 1 increased SM specific transcripts in a dose dependent manner. TGF beta 1 proteins were detected in amniotic fluid at a time when the intestine was physiologically herniated. By analogy with the requirement for exogenous TGF beta 1 for SM differentiation in organ culture, the TGF beta 1 protein that was demonstrated to be present in the amniotic fluid may enhance intestinal development when it is physiologically herniated in early gestation. Future studies of embryonic intestinal cultures should include TGF beta 1 in the defined media to produce a more faithful model of in vivo muscle differentiation. Copyright (c) 2017 The Authors Journal of Tissue Engineering and Regenerative Medicine Published by John Wiley & Sons, Ltd
机译：在理论上将在体内肠开发中复制的前体内实验策略提供了学习正常和多重肠道生物学的可访问设置。目前作者最近描述了一种系统，其中将小鼠胚胎Jejunal段介绍在半透平台上并用化学定义的无血清培养基。在器官文化中超过3天，外植体形成绒毛，他们开始接受自发的蠕动。如目前的研究中所定义的，外植入肠道的壁未能形成稳健的纵向平滑肌（SM）层，因为它在同一时间段内将在体内进行。鉴于将生长因子β1（TGFβ1）转化在其他器官中的SM分化中的作用，假设外源TGFβ1将增强这些外植体的SM分化。在体内，TGFβ受体I和II均在胚胎纵向Jejunal SM细胞中检测，并且在器官培养中，外源TGFβ1诱导纵向SM的稳健分化。微阵列分析显示TGFβ1以剂量依赖性方式增加SM特异性转录物。在肠道在生理突出的时候，在羊水中检测到TGFβ1蛋白。通过类比与器官培养中的SM分化的外源TGFβ1的要求，当在早期妊娠时生理突出时，表明存在于羊水中的TGFβ1蛋白可能增强肠道发育。未来对胚胎肠培养的研究应包括定义介质中的TGFβ1，以产生更忠实的体内肌肉分化模型。作者：王莹，王莹，王莹，王莹，王莹，王莹，王莹

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号