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首页> 外文期刊>Journal of tissue engineering and regenerative medicine >Full-thickness oesophageal regeneration in pig using a polyurethane mucosal cell seeded graft
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Full-thickness oesophageal regeneration in pig using a polyurethane mucosal cell seeded graft

机译：使用聚氨酯粘膜细胞种子移植物的猪中全厚的食管再生

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Malignant oesophageal pathology typically requires resection of a portion of oesophagus. The aim of this study was to investigate attachment and growth of swine oesophageal mucosal cells on electrospun synthetic nanofibre matrices of varying chemistries and to determine whether a mucosal-seeded graft, in a swine animal model, could induce regeneration. Swine mucosal oesophageal cells were isolated and seeded them onto five different matrix materials. Matrix samples were cultured for up to 14days, after which matrices were analysed for cell attachment. Attachment varied for each of the matrix materials tested, with the most rigid showing the lowest levels of attachment. Importantly, sections of these matrices illustrated that multiple layers of mucosal cells formed, mimicking endogenous oesophageal structure. A tdTomato reporter line (mucosal(tdt) cells) was created to enable cell tracking. As polyurethane matrix was found optimal through in vitro testing, a graft was prepared using mucosal(tdt) cells, along with an unseeded control, and implanted into swine for determination of oesophageal regeneration. Mucosal seeded polyurethane grafts initiated full thickness regeneration of the oesophagus, including epithelial, submucosal, and skeletal muscle layers which were highly vascularized. Interestingly, an unseeded graft showed similar regeneration, indicating that the role of cells in the process of oesophageal regeneration is still unclear. The electrospun polyurethane matrix does appear suitable for multilayered cellular attachment and growth of oesophageal mucosal cells, and implantation of polyurethane grafts initiated full thickness regeneration of the oesophagus, indicating potential for oesophageal reconstruction in humans. Copyright (C) 2016 John Wiley & Sons, Ltd.
机译：恶性食管病理学通常需要切除一部分食道。本研究的目的是研究猪食管粘膜细胞对电纺织乳膜细胞对不同化学物质的合成纳米纤维矩阵的附着和生长，并确定猪动物模型中粘膜上苗接枝是否可以诱导再生。将猪粘膜切割细胞分离并将它们接种到五种不同的基质材料上。将基质样品培养至14天，之后分析了细胞附着的基质。对于测试的每个基质材料而变化，具有最刚性的最刚性的附件。重要的是，这些基质的部分示出了形成多层形成的粘膜细胞，模拟内源性食管结构。创建TDTOMATO报告线（粘膜（TDT）细胞）以实现细胞跟踪。由于通过体外测试发现聚氨酯基质最佳，使用粘膜（TDT）细胞，以及未发现的对照，并植入猪的猪肉再生成衍生物，以确定猪肉再生。粘膜种子聚氨酯移植物引发了食道的全厚度再生，包括高度血管化的上皮，粘膜和骨骼肌层。有趣的是，一个未化的移植物显示出类似的再生，表明细胞在食管再生过程中的作用尚不清楚。静电梭聚氨酯基质表现出适用于多层细胞附着和食管粘膜细胞的生长，并且聚氨酯移植物的植入引发了食道的全厚度再生，表明人类中的食管重建潜力。版权所有（c）2016 John Wiley＆Sons，Ltd。

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