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首页> 外文期刊>Journal of tissue engineering and regenerative medicine >Generation of full-thickness skin equivalents using hair follicle-derived primary human keratinocytes and fibroblasts
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Generation of full-thickness skin equivalents using hair follicle-derived primary human keratinocytes and fibroblasts

机译：使用毛发卵泡衍生的原发性人角蛋白细胞和成纤维细胞产生全厚皮肤等同物

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摘要

Skin equivalents are increasingly used as human-based test systems for basic and preclinical research. Most of the established skin equivalents are composed of primary keratinocytes and fibroblasts, isolated either from excised human skin or juvenile foreskin following circumcisions. Although the potential of hair follicle-derived cells for the generation of skin equivalents has been shown, this approach normally requires microdissections from the scalp for which there is limited subject compliance or ethical approval. In the present study, we report a novel method to isolate and cultivate keratinocytes and fibroblasts from plucked hair follicles that were then used to generate skin equivalents. The procedure is non-invasive, inflicts little-pain, and may allow easy access to patient-derived cells without taking punch biopsies. Overall, minor differences in morphology, ultrastructure, expression of important structural proteins, or barrier function were observed between skin equivalents generated from hair follicle-derived or interfollicular keratinocytes and fibroblasts. Interestingly, improved basal lamina formation was seen in the hair follicle-derived skin equivalents. The presented method here allows easy and non-invasive access to keratinocytes and fibroblasts from plucked hair follicles that may be useful particularly for the generation of skin disease equivalents.
机译：皮肤等同物越来越多地用作基于人的基础和临床前研究的测试系统。大多数已建立的皮肤等同物由原代角蛋白细胞和成纤维细胞组成，分离出在包围后切除的人体皮肤或少年包皮。尽管已经显示了用于产生皮肤等同物的毛囊衍生细胞的潜力，但这种方法通常需要来自头皮的微小体，其中有受限的受试者合规或道德批准。在本研究中，我们报告了一种新的方法，以分离和培养角蛋白细胞和成纤维细胞从拔除毛囊中培养，然后用于产生皮肤等同物。该程序是非侵入性的，造成很小疼痛，并且可以轻松地进入患者衍生的细胞而不服用打孔活组织检查。总体而言，在从毛囊衍生或间隙角细胞和成纤维细胞产生的皮肤等同物之间观察到形态学，超微结构，重要结构蛋白的表达或阻隔功能的微小差异。有趣的是，在毛囊衍生的皮肤等同物中看到改善的基底层形成。本发明的方法可以易于易于和非侵入性进入从拔牙卵泡的角质形成细胞和成纤维细胞，其可用于产生皮肤病当量的产生。

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