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首页> 外文期刊>Journal of tissue engineering and regenerative medicine >Effectiveness of tissue engineered three-dimensional bioactive graft on bone healing and regeneration: an in vivo study with significant clinical value
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Effectiveness of tissue engineered three-dimensional bioactive graft on bone healing and regeneration: an in vivo study with significant clinical value

机译：组织工程三维生物活性移植物对骨愈合和再生的有效性：体内研究具有重要临床价值

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Several strategies have been used to promote bone repair, with many failing due to the lack of osteoinduction. This report describes an approach for promoting bone healing that attempts to overcome prior shortcomings. First, the role was compared of different concentrations of gelatine (Gel), nanostructured-hydroxyapatite (nHA), simvastatin (Sim) and nHA-Sim particles on healing of small femoral bone defects in rabbits. The effective concentration of each was studied, and then a three-dimensional porous scaffold was designed using Gel, nHA and Sim, which was then cross-linked with genipin. Morphology, degradation profile and Sim delivery properties of the scaffolds were evaluated in vitro. Then, the scaffolds were subcutaneously tested in vivo to determine their biocompatibility, biodegradability and osteogenic properties. Finally, the scaffolds were implanted in a large radial bone defect model in rabbits and their effect on bone regeneration was investigated. The Gel, nHA and Sim with concentrations of 1, 1 and 5 mg/femoral hole were effective during bone healing respectively, and the Sim showed the most osteoinduction and osteoconduction when compared to controls. The Gel-Sim and Gel-nHA-Sim scaffolds continuously and homogenously released Sim into the simulated body fluid in vitro. Subcutaneously, the scaffolds were biocompatible, biodegradable and able to produce ectopic bone after 30 days. Thirty and 60 days after implantation of the scaffolds in radial bone defects, they were completely degraded and replaced with the new bone that had significantly superior morphology, mineral density, bioelectrical, biophysical and micromechanical properties compared with controls. Such bioactive grafts may be a suitable option for bone reconstruction, healing and repair.
机译：若干策略已被用来促进骨骼修复，由于缺乏骨髓丁基，许多失败。本报告描述了一种促进骨骼治疗，试图克服现有缺点的方法。首先，将作用与不同浓度的明胶（凝胶），纳米结构 - 羟基磷灰石（NHA），辛伐他汀（SIM）和NHA-SIM颗粒进行比较，请参见兔子小股骨骨缺损的愈合。研究了各自的有效浓度，然后使用凝胶，NHA和SIM设计三维多孔支架，然后与Genipin交联。在体外评估支架的形态，降解曲线和SIM递送性能。然后，在体内皮下测试支架以确定它们的生物相容性，生物降解性和成骨特性。最后，将支架植入兔中的大径向骨缺陷模型中，并研究了它们对骨再生的影响。凝胶，NHA和浓度为1,1和5mg /股骨孔的骨骼愈合过程中有效，与对照相比，SIM显示出最骨诱导和骨质化。将凝胶 - SIM和凝胶-NHA-SIM支架在体外连续和均匀地将SIM释放到模拟体液中。皮下，支架是生物相容性的，可生物降解的，并且能够在30天后产生异位骨。在径向骨缺损中植入支架后的三十和60天，它们完全降解并用对照组具有显着优异的形态，矿物密度，生物电，生物物理和微机械性能的新骨。这种生物活性移植物可以是骨重建，愈合和修复的合适选择。

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