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首页> 外文期刊>Journal of tissue engineering and regenerative medicine >Preparation of cell aggregates incorporating gelatin hydrogel microspheres of sugar-responsive water solubilization
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Preparation of cell aggregates incorporating gelatin hydrogel microspheres of sugar-responsive water solubilization

机译：含有明胶水凝胶微球的细胞聚集体的制备

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摘要

The objective of this study is to design hydrogel microspheres of a cell scaffold, which not only function as a scaffold to form cell aggregates of three-dimensional culture but also can disappear to release growth factors in the well-controlled manner by noncytotoxic stimulation in any timing. The hydrogel microspheres were prepared by a water-in-oil emulsion method fromm-aminophenylboronic acid (APBA)-introduced gelatin (APBA-gelatin) with or without poly(vinyl alcohol) (PVA) mixing. Irrespective of the PVA concentration, the microspheres with the same diameter were prepared. The microspheres were water solubilized only by adding sorbitol of a sugar although the solubilization extent depended on the PVA concentration. When cocultured with the microspheres, mesenchymal stem cells formed cell aggregates homogeneously incorporating the microspheres. Upon adding sorbitol in the culture medium, mixed APBA-gelatin-PVA hydrogel microspheres disappeared with time in the cell aggregates. The microspheres containing basic fibroblast growth factor or bone morphogenetic protein-2 released the respective growth factor accompanied with the microspheres disappearance. It is concluded that the present microspheres of sugar-responsive water solubilization are promising scaffold of cell aggregates and have an ability to allow growth factors to be released in the cell aggregates when it is required.
机译：本研究的目的是设计细胞支架的水凝胶微球，其不仅用作支架以形成三维培养的细胞聚集体，而且可以在任何非胞素毒性刺激中消失以释放良好控制的方式的生长因子定时。水凝胶微球通过水 - 氨基苯基硼酸（APBA） - 靛蓝蛋白（APBA） - 荧光蛋白（APBA-明胶）制备，具有或不具有聚（乙烯醇）（PVA）混合。不管PVA浓度如何，制备具有相同直径的微球。虽然溶解度依赖于PVA浓度，但微球仅通过添加糖的山梨糖醇溶解。当用微球和微球和分配时，间充质干细胞形成细胞聚集体均匀地掺入微球。在培养基中添加山梨糖醇后，混合APBA-明胶-PVA水凝胶微球随着细胞聚集体的时间消失。含有碱性成纤维细胞生长因子或骨形态发生蛋白-2的微球释放出伴随微球消失的各自的生长因子。得出结论，糖响应水溶性的目前微球是电池聚​​集体的承受支架，并且在需要时允许允许在细胞聚集体中释放生长因子的能力。

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