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首页> 外文期刊>Journal of Veterinary Diagnostic Investigation >Validation of a commercial magnetic bead-based multiplex assay for 5 novel biomarkers of acute kidney injury in canine serum
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Validation of a commercial magnetic bead-based multiplex assay for 5 novel biomarkers of acute kidney injury in canine serum

机译：在犬血清急性肾损伤的5个新型生物标志物的验证验证犬血清急性肾脏损伤

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摘要

Interest is growing in measurement of novel biomarkers for the diagnosis of acute kidney injury. Multiplex assays may provide a rapid and cost-effective way of measurement; however, sparse information is published regarding their use in dogs. We aimed to validate a commercial magnetic bead-based assay for 5 biomarkers: clusterin (Clus), cystatin C (CysC), kidney injury molecule 1 (KIM-1), monocyte chemoattractant protein 1 (MCP-1), and neutrophil gelatinase-associated lipocalin (NGAL). Intra- and inter-assay imprecision, linearity under dilution (LUD), spike recovery (S-R), and hemoglobin interference were evaluated using serum from healthy and diseased dogs. Additionally, the effect of sample type (serum vs. plasma) was investigated. All values for Clus and MCP-1 were outside the assay's measurable range. Intra- and inter-assay precision were acceptable for NGAL (CVs 8.8% and 13.2%, respectively). Regression analysis of LUD and S-R indicated good linearity for CysC and NGAL. Hemolysis did not affect measurement of any biomarker. Measured concentrations of CysC (p= 0.018) and NGAL (p= 0.015) were significantly lower in sodium citrate plasma compared to serum. We conclude that this magnetic bead-based assay is precise and accurate for NGAL measurement in canine serum. Inappropriate standards for MCP-1 and Clus, and poor accuracy for KIM-1 measurement, suggest that this assay cannot reliably quantify those biomarkers in canine blood. Measurements of CysC in canine blood using this assay must be interpreted with caution given inter-assay imprecision.
机译：兴趣在测量新型生物标志物中的诊断急性肾损伤的兴趣。多重测定可以提供快速且经济高效的测量方式;但是，稀疏的信息是关于他们在狗中使用的。我们的目标是为了验证5个生物标志物的商业磁珠基测定：Clerialin（Clus），胱抑素C（Cysc），肾损伤分子1（Kim-1），单核细胞化学抑制剂蛋白1（MCP-1）和中性粒细胞明胶酶 - 相关的脂素（Ngal）。使用来自健康和患病的狗的血清评估稀释（LUD），穗恢复（S-R），穗恢复（S-R）和血红蛋白干扰的线性度。另外，研究了样品型（血清血清）的效果。 CLU和MCP-1的所有值都在测定范围之外。对于NGAL（CVS 8.8％和13.2％）可接受测定和测定型精度。 LUD和S-R的回归分析表明CYSC和NGAL的良好线性度。溶血不影响任何生物标志物的测量。与血清相比，测量的CySC（P = 0.018）和Ngal（P = 0.015）的浓度显着降低柠檬酸钠等离子氨酸钠。我们得出结论，这种基于磁珠的测定对于犬血清中的Ngal测量精确和准确。 MCP-1和Clus的不适当标准，Kim-1测量的准确性差，表明该测定不能可靠地量化犬血中的那些生物标志物。使用该测定的犬血液中的Cysc测量必须在测定间不精确的情况下小心解释。

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