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首页> 外文期刊>Journal of Veterinary Research >Effect of pregnancy and stage of lactation on energy processes in isolated blood cells of dairy cows
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Effect of pregnancy and stage of lactation on energy processes in isolated blood cells of dairy cows

机译：妊娠与诉讼阶段对奶牛分离血细胞能源过程的影响

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Introduction: The transition period is the most challenging time for dairy cattle, which is characterised not only by negative energy balance but also by fatty tissue mobilisation. Material and Methods: The efficiency of energy pathways, beta-oxidation in WBC and glycolysis in RBC (based on deoxyglucose transmembrane transport) were estimated. Insulin in blood plasma was determined using ELISA. Results: After calving and up to one month after delivery, a significant drop in blood plasma level was noticed, simultaneously with a rise in beta-oxidation from 18.93 +/- 3.64 to 30.32 +/- 5.28 pmol/min/mg protein in WBC. A strong negative correlation between these two indices (r = -0.68) was found. During the period of transition to lactation an increase in glucose cross-membrane transportation from 41.44 +/- 4.92 to 50.49 +/- 6.41 mu mol/h/g Hb was observed. A strong positive correlation between glucose transportation in RBC and beta-oxidation in WBC (r = 0.71) was noticed. These data are in agreement with results of studies on dairy cows using liver slices from dairy cows in late pregnancy and different stages of lactation, in which changes in gene expression were analysed. Conclusion: It seems that measuring fatty acids oxidation and glycolysis using isolated blood cells may be an adequate and relatively simple method for energy state analysis to estimate the state of dairy cow metabolism and animal health.
机译：介绍：过渡期是奶牛最具挑战性的时间，这不仅是由负能量平衡而且通过脂肪组织调动的特征。估计了材料和方法：估计了对RBC（基于脱氧葡萄糖跨膜运输）的WBC中的能量途径，β-氧化和糖酵解的效率。使用ELISA测定血浆中的胰岛素。结果：递送后持续至多一个月后，注意到血浆水平的显着下降，同时β-氧化在WBC中的18.93 +/- 3.64至30.32 +/- 5.28 pmol / min / mg蛋白质同时。发现这两个索引之间的强度负相关（R = -0.68）。在过渡期泌乳期间，观察到41.44 +/- 4.92至50.49 +/-6.41μmmol/ h / g Hb的葡萄糖跨膜运输的增加。注意到，WBC（R = 0.71）中RBC和β-氧化的葡萄糖运输与β-氧化之间的强阳性相关性。这些数据与乳制品奶牛的研究结果一致，使用乳制奶牛在妊娠晚期和哺乳期的不同阶段的乳制奶牛，分析了基因表达的变化。结论：似乎使用分离血细胞测量脂肪酸氧化和糖酵解可能是能源状态分析的足够和相对简单的方法，以估算奶牛代谢和动物健康状况。

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