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首页> 外文期刊>Journal of Veterinary Research >Improvement of sperm motility within one month under selenium and vitamin E supplementation in four infertile dogs with low selenium status
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Improvement of sperm motility within one month under selenium and vitamin E supplementation in four infertile dogs with low selenium status

机译：在硒和维生素E补充硒的一个月内的精子活力的改善

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Introduction: Significant improvement of sperm motility within one month effected by oral supplementation of selenium and vitamin E was described in four infertile male dogs which failed to conceive in their last three matings with different bitches. Material and Methods: The dogs (a Golden Retriever, an English Cocker Spaniel, and two Tibetan Mastiffs) were supplemented daily with selenium (Se) (0.6 mg/kg organic Se yeast) and vitamin E (vit. E) (5 mg/kg) per os for 60 days. Semen was collected on days 0, 30, 60, and 90. The sperm concentration and motility parameters were evaluated by the CASA system, sperm morphology was explored by Diff-Quick staining, and live and dead spermatozoa were differentiated by eosin/nigrosin staining. The concentrations of Se and vit. E were measured in peripheral blood serum on semen collection days. Results: Before administration, the concentrations of Se in blood plasma were low (86.0-165.0 mu g/L). After 30 days of treatment there was an observable improvement in total and progressive sperm motility and kinematic parameters (VAP, VSK, VCL, ALH, BCF, and RAPID). The percentages of live and normal morphology sperm cells were also higher. There was also an observable increase in Se and vitamin E concentrations in blood serum. Bitches were successfully mated and delivered four to six puppies. Conclusion: Supplementation with Se and vit. E improved rapid sperm motility and restored fertility in infertile dogs with low Se status.
机译：介绍：在四种不孕雄性狗中描述了由口服补充硒和维生素E的一个月内的精子活性的显着改善，该幼犬未能在其最后三个带有不同的母狗的情况下构思。材料和方法：狗（金猎犬，英国可卡犬和两个藏獒）每日补充硒（0.6mg / kg有机Se酵母）和维生素E（Vit.e）（5毫克/ kg）每次操作60天。在第0,30,60和90天收集精液。通过CASA系统评估精子浓度和运动参数，通过差异快速染色探索的精子形态，并且通过eosin / nigrosin染色来分化活的生物和死子。 SE和VIT的浓度。 E在精液血清中测量精液收集天。结果：给药前，血浆中SE的浓度低（86.0-165.0μg/ l）。 30天治疗后，总和的精子运动和运动学参数（VAP，VSK，VCL，ALH，BCF和RAPP）患有可观察到的改善。活性和正常形态细胞的百分比也更高。血液血清中的SE和维生素E浓度也有可观察到的。母狗成功地交配并交付了四到六只小狗。结论：通过SE和VIT进行补充。 e改善了具有低SE状态的不育犬的快速精子运动和恢复生育能力。

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