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首页> 外文期刊>Journal of Veterinary Research >Cloning and identification of PK15 cells for enhanced replication of classical swine fever virus
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Cloning and identification of PK15 cells for enhanced replication of classical swine fever virus

机译：PK15细胞增强复制典型猪瘟病毒的克隆及鉴定

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Introduction: Classical swine fever virus (CSFV) causes an economically important and highly contagious disease of pigs, leading to economic losses around the world. Attenuated live vaccines with CSFV antigens have played an important role in the prevention and control of the disease. Porcine kidney 15 (PK15) cells have been widely used for the propagation of CSFV, but this cell line is not efficient or homogeneously susceptible to viral infection. Material and Methods: To achieve a homogeneous PK15 cell line which enabled high titre replication of CSFV, we used the limiting dilution cell cloning method. Results: We developed two cell clones, PK15-1A6 and PK15-3B1, which respectively have high- and low-permissive phenotypes to CSFV infection. The PK15-1A6, PK15-3B1, and PK15 parent cells showed different characteristics in cell proliferation rate, susceptibility to CSFV infection, and CSFV production. The mean virus titres per millilitre reflected by TCID50 values in PK15-1A6, PK15-3B1, and PK15 parent cells were 10(6.85), 10(3.63), and 10(4.74), respectively. Conclusion: The PK15-1A6 cell clone is more permissive to CSFV infection than the PK15 parent cells. The screened high-permissive cells will be useful for CSFV propagation and vaccine development in vitro, and facilitate research on the pathogenicity of CSFV.
机译：简介：古典猪瘟病毒（CSFV）导致经济上重要和高度传染性的猪，导致世界各地的经济损失。具有CSFV抗原的减毒活疫苗在预防和控制疾病中发挥了重要作用。猪肾15（PK15）细胞已广泛用于CSFV的繁殖，但这种细胞系不高效或均匀地易于病毒感染。材料和方法：为了实现使CSFV的高滴度复制的均匀PK15细胞系，我们使用了限制稀释电池克隆方法。结果：我们开发了两个细胞克隆，PK15-1A6和PK15-3B1，其分别对CSFV感染具有高且低允许的表型。 PK15-1A6，PK15-3B1和PK15亲本细胞显示细胞增殖率，对CSFV感染的易感性以及CSFV产生的不同特征。 PK15-1A6，PK15-3B1和PK15亲本细胞中TCID50值反射的每毫升的平均病毒滴度分别为10（6.85），10（3.63）和10（4.74）。结论：PK15-1A6细胞克隆对CSFV感染更依赖于PK15亲本细胞。筛选的高允许细胞对于体外CSFV繁殖和疫苗发育可用于CSFV繁殖和疫苗开发，并促进CSFV致病性的研究。

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