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首页> 外文期刊>Journal of Veterinary Research >Prevalence and phylogenetic analysis of Mycoplasma synoviae strains isolated from Polish chicken layer flocks
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Prevalence and phylogenetic analysis of Mycoplasma synoviae strains isolated from Polish chicken layer flocks

机译：从波兰鸡层群中分离的支原体Synoviae菌株的患病率和系统发育分析

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Introduction: Mycoplasma synoviae (MS) is a chicken pathogen of major economic importance. Material and Methods: Between 2010 and 2016, 906 commercial layer chicken flocks in Poland were examined for MS, and the phylogenetic relationship among the strains was established. Regionally dispersed samples were collected and tested with the use of real-time PCR to detect the 16S-23S intergenic spacer region. Positive samples were also tested with LAMP and conventional PCR to detect the vlhA gene. Results: MS genetic material was detected in 265 (29%) of the tested flocks by real-time PCR, in 227 by the LAMP method and in 202 (22%) by conventional PCR. The by-year percentage of positive samples began at 34% in 2010, rose to 44% in 2012, and declined to 29% in 2016. A phylogenetic analysis of Polish M. synoviae strains using a partial sequence of the vlhA gene showed nine genotypes (A-I), the most frequently occurring being F and C. Pathogenic Polish MS field isolates (n = 27) collected from chickens with clinical signs of infection were grouped for their characteristic symptoms: respiratory for genotypes C, E, F, and I (n = 13), EAA and a drop in laying for genotypes F, E, and C (n = 12), and synovitis for genotype A (n = 2). Conclusion: These data showed the country's isolate diversity. The high prevalence suggests the need to introduce appropriate control programmes. This is the first report of molecular epidemiological data on M. synoviae infection in layer chickens in Poland.
机译：介绍：支原体Synoviae（MS）是一种主要经济意义的鸡病病原疮。材料和方法：2010年和2016年间，对Poland的906个商业层鸡群进行检查，并建立了菌株之间的系统发育关系。收集区域分散的样品并使用实时PCR检测16S-23S代轴间隔区。还用灯和常规PCR测试阳性样品以检测VLHA基因。结果：通过实时PCR在265（29％）的测试植物中检测MS遗传物质，通过灯法，通过常规PCR在202（22％）中，在227中检测到265（29％）。积极样本的百分比百分比于2010年开始34％，2012年上涨至44％，并在2016年下降至29％。使用VLHA基因的部分序列的波兰M.Sogoviae菌株的系统发育分析显示为九种基因型（AI），从鸡群中收集的最常发生的F和C.致病性抛光MS场分离株（n = 27）与感染临床迹象进行分组，用于其特征症状：基因型C，E，F和I（ n = 13），EAA和铺设基因型F，E和C（n = 12）的下降，以及基因型A的滑膜炎（n = 2）。结论：这些数据显示该国的孤立多样性。高流行率表明需要引入适当的控制计划。这是波兰层鸡中M. Sogoviae感染的分子流行病学数据的第一报告。

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