• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Journal of Veterinary Research >Real-time loop-mediated isothermal amplification (LAMP) of mgc2 gene of Mycoplasma gallisepticum
【24h】

Real-time loop-mediated isothermal amplification (LAMP) of mgc2 gene of Mycoplasma gallisepticum

机译：支原体MgC2基因的实时环介导等温扩增（灯）

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Introduction: Mycoplasma gallisepticum is considered the most pathogenic and economically significant avian Mycoplasma spp. for the worldwide poultry industry. The aim of this study was to develop a novel and sensitive real-time loop-mediated isothermal amplification (LAMP) assay based on the amplification of its mgc2 gene sequence for its rapid molecular detection in poultry. Material and Methods: Blood samples from 300 broiler and layer chickens were screened using a rapid serum agglutination (RSA) test. A real-time LAMP reaction was conducted with seropositive swab samples at 60 degrees C for 90 min in an ESEQuant tube scanner using 6-carboxyfluorescein as the reporting dye. Results: The sensitivity of the developed assay was 10 fg/mu L of DNA. The assay was found 100% specific, showing no cross-reactivity with other avian Mycoplasma species. The proportion found of the positive samples by the real-time LAMP was 58%. In comparison, the RSA was found to detect 52% of positive cases. Conclusion: The mgc2 real-time LAMP emerged as a more sensitive and accurate method for molecular detection of M. gallisepticum than RSA. Robustness and precision give it applicability as a potential field diagnostic tool for M. gallisepticum control. The study will be beneficial in reducing economic losses that M. gallisepticum inflicts on the poultry industry. This is the first reported development of a real-time LAMP assay based on the amplification of the mgc2 gene sequence using an ESEQuant tube scanner for galline M. gallisepticum detection.
机译：介绍：支原体Gallisepticum被认为是最致病性和经济上有明显的禽架支原体SPP。为全球家禽行业。本研究的目的是基于其MGC2基因序列的扩增，在家禽中的快速分子检测中，开发一种新颖和敏感的实时环介导等温扩增（灯）测定。材料和方法：使用快速血清凝集（RSA）试验筛选来自300个肉鸡和层鸡的血液样品。使用6-羧基荧光素作为报告染料，在60℃下在60℃下在60℃下在60℃下以60℃进行血清阳性拭子样品进行实时灯反应。结果：开发测定的敏感性为10fg / mu L的DNA。发现该测定法具体，表现出与其他禽老性的支原体物种的交叉反应性。实时灯的阳性样品的比例为58％。相比之下，发现RSA检测阳性病例的52％。结论：MGC2实时灯作为M. gallisepticum的分子检测更敏感和准确的方法。鲁棒性和精度使其适用于M. gallisepticum控制的潜在现场诊断工具。该研究将有利于降低M. gallisepticum在家禽业造成的经济损失。这是第一次报道基于使用Esquant管扫描仪进行Galline M. gallisepticum检测的MGC2基因序列的扩增的实时灯测定的开发。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号