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首页> 外文期刊>Journal of Veterinary Research >Application of the AhR reporter gene assay for the determination of PCDD/Fs and DL-PCBs in feed samples
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Application of the AhR reporter gene assay for the determination of PCDD/Fs and DL-PCBs in feed samples

机译：AHR报告基因测定在饲料样品中测定PCDD / FS和DL-PCB的应用

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Introduction: Polychlorinated dibenzo-p-dioxins, polychlorinated dibenzofurans (PCDD/Fs) and dioxin-like polychlorinated biphenyls (DL-PCBs) belong to a well-known group of pollutants. Present in feedstuffs, they bioaccumulate in tissues of food-producing animals. Food is the source of over 90% of human PCDD/Fs and DL-PCBs intake. Thus, feed control is one of the pillars of the EU strategy and a mean of reducing human exposure. The article presents AhR based reporter gene bioassay method for PCDD/Fs and DL-PCBs analysis in feed and its validation results. Material and Methods: Analytes were extracted from samples with fat. Subsequently, fat and other interferences were removed from extract using sulphuric acid modified silica. Extract was further cleaned and PCDD/Fs separated from DL-PCBs using carbon column. Contaminants detection was performed using H1L6.1c3 cell line, which produces luciferase in response to AhR ligands present in extract. Results: Performance characteristics (repeatability, reproducibility, and apparent recovery) fulfil the requirements of Regulation 2017/771/EU. The positive correlation between bioassay and reference HRGC-HRMS method was confirmed. Moreover, the role of screening method used in connection with the confirmatory HRGC-HRMS method in providing feed and food safety has been discussed. Conclusion: Bioassay is a useful method for dioxin and DL-PCBs analysis, allowing cost reduction of monitoring programmes with minimal risk of false negative results.
机译：介绍：多氯二苯苄硫氧化锡，聚氯二苯并呋喃（PCDD / FS）和二恶英的多氯联苯（DL-PCB）属于着名的污染物组。饲料中存在，它们在食品动物组织中生物累积。食物是超过90％的人PCDD / FS和DL-PCB Intake的来源。因此，进料控制是欧盟策略的柱之一，以及减少人类暴露的平均值。本文介绍了饲料中PCDD / FS和DL-PCB分析的基于AHR的报告基因生物测定方法及其验证结果。材料和方法：分析物从具有脂肪的样品中提取。随后，使用硫酸改性二氧化硅从提取物中除去脂肪和其他干扰。进一步清洁提取物，使用碳柱与DL-PCB分离的PCDD / FS。使用H1L6.1C3细胞系进行污染物检测，其产生响应于提取物中的AHR配体的荧光素酶。结果：性能特征（可重复性，再现性和表观恢复）履行2017/771 /欧盟的规定要求。证实了生物测定与参考HRGC-HRMS方法之间的正相关性。此外，已经讨论了结合验证性HRGC-HRMS方法提供提供饲料和食品安全性的筛选方法的作用。结论：生物测定是二恶英和DL-PCB分析的有用方法，允许降低监测程序，具有最小的假阴性结果风险。

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