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Screening for circulating miR-208a and -b in different cardiac arrhythmias of dogs

机译：筛选循环miR-208a和-b在狗的不同心律失常中

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Introduction: In recent years, the high sensitivity and specificity of novel miRNA biomarkers have been utilised for early diagnosis and treatment monitoring of various diseases. Previous reports showed that abnormal expression of miR-208 in mice resulted in the development of an aberrant cardiac conduction system and consecutive arrhythmias. On the other hand, a study on infarcted human heart tissue showed upregulation of miR-208a in subjects with ventricular tachyarrhythmias compared to healthy controls. We prospectively investigated the expression of miR-208a and -208b in the serum of dogs presenting different cardiac arrhythmias. Material and Methods: A total of 28 dogs with atrial fibrillation (n = 8), ventricular premature contractions (n=6), conduction system disturbances (n = 7), and free of heart conditions (as controls) (n = 7) were enrolled in the study. Total RNA was extracted from serum samples and miR-208a and -b, miR-16 as well as a cel-miR-39-5p spike-in were analysed with qPCR and ddPCR. Results: miR-208a and miR-208b were not expressed in any of the samples. The calculated ddPCR miR-16 relative expression (normalised with cel-miR-39 spike-in) showed a good correlation (r = 0.82; P 0.001) with the qPCR results. Conclusion: This outcome warrants further investigation, possibly focusing on tissue expression of miR-208 in the canine heart.
机译：介绍：近年来，新型miRNA生物标志物的高敏感性和特异性已被用于各种疾病的早期诊断和治疗监测。之前的报道显示，小鼠MiR-208的异常表达导致开发异常的心脏传导系统和连续的心律失常。另一方面，与健康对照相比，对梗死的人体心脏组织的研究显示了室性心律失常的受试者中miR-208a的上调。我们预期研究了患有不同心律失常的狗血清中miR-208a和-208b的表达。材料和方法：共28只具有心房颤动的犬（n = 8），心室过早收缩（n = 6），传导系统紊乱（n = 7），没有心脏病（作为对照）（n = 7）注册了这项研究。用QPCR和DDPCR从血清样品和-MIR-208A和-B，MIR-16以及-MIR-39-5P峰值中提取总RNA和-B，MIR-39-5P峰值。结果：MIR-208A和MIR-208B未在任何样品中表达。计算的DDPCR miR-16相对表达（用CEL-miR-39峰值刺激）显示出QPCR结果的良好相关性（r = 0.82; p <0.001）。结论：此结果需要进一步调查，可能关注MiR-208在犬心中的组织表达。

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