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首页> 外文期刊>Journal of Veterinary Research >Lack of association between Epstein-Barr virus and mammary tumours in dogs
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Lack of association between Epstein-Barr virus and mammary tumours in dogs

机译：狗缺乏盲肠 - 巴尔病毒与狗的乳腺肿瘤之间的关联

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Introduction: Epstein-Barr virus (EBV) is a gamma-herpesvirus associated with various neoplasms in humans and is a probable aetiological agent in breast cancer; however, a causal relationship has not yet been established. Because of the epidemiological and clinicopathological similarities between breast cancer and canine mammary tumours, dogs have been proposed as a valid model for breast cancer. Material and Methods: A total of 47 canine mammary gland tumour tissues were processed by routine histopathological technique with haematoxylin-eosin staining and classified according to the type of neoplasm. DNA was extracted from paraffm-embedded tissues and the EBNA-1 gene and the BamHI-W region specific for EBV were evaluated by nested PCR. Results: The histopathological evaluation revealed 2 benign neoplasms, and many carcinomas: 2 in situ, 9 simple, 3 solid, 10 complex, and 21 mixed. One sample was positive for the EBNA-1 gene, while all were negative for the BamHI-W region. Conclusion: No association was found between EBV and mammary tumours in dogs. However, here we report for the first time the presence of an EBV gene sequence in a canine mammary tumour. It is likely that detection of EBV might be affected by the quality and quantity of DNA extracted from paraffm-embedded tissues. Additional studies are necessary to establish any association of EBV with mammary gland cancer in humans and in dogs, which could eventually lead to better public health prevention and control.
机译：简介：Epstein-Barr病毒（EBV）是一种与人类各种肿瘤相关的γ-疱疹病毒，是乳腺癌中可能的Aetiological;但是，尚未建立任何因果关系。由于乳腺癌和犬哺乳动物肿瘤之间的流行病学和临床病理学相似性，已提出狗作为乳腺癌的有效模型。材料和方法：通过常规组织病理学技术用Haematyxylin-eosin染色和根据肿瘤的类型进行分类，共处理47只犬氨基乳腺肿瘤组织。通过嵌套的PCR从Paraffc嵌入式组织中提取DNA，并通过嵌套的PCR评估EBN-1基因的BABI-W.特异的BAMHI-W区域。结果：组织病理学评价显示出2份良性肿瘤，以及许多癌：2原位，9，3固体，10个络合物，21个混合。一个样品对于EBNA-1基因是阳性的，而BamHI-W区域则均为阴性。结论：在狗的EBV和乳腺肿瘤之间没有发现任何关联。然而，在这里，我们首次报告犬乳腺肿瘤中的EBV基因序列的存在。 eBV的检测可能会受到从原体嵌入式组织中提取的DNA的质量和量的影响。额外的研究是在人类和狗的任何乳腺癌中建立任何与乳腺癌的联系，最终可能导致更好的公共卫生预防和控制。

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