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首页> 外文期刊>Journal of Veterinary Research >Genetic analysis of the M gene of equine influenza virus strains isolated in Poland, in the context of the Asian-like group formation
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Genetic analysis of the M gene of equine influenza virus strains isolated in Poland, in the context of the Asian-like group formation

机译：在波兰中分离出的马流感病毒菌株M基因的遗传分析，在亚洲群组形成的背景下

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Introduction: Traditionally, evolutionary analysis of equine influenza virus (EIV) is based on the HA gene. However, the specificity of the influenza virus enables the classification of viral strains into different phylogenetic groups, depending on the gene being analysed. The aim of the study was to analyse phylogenetic paths of EIV based on M gene with reference to the HA gene. Material and Methods: M gene of Polish isolates has been sequenced and analysed along with all M sequences of EIV available in GenBank database. Phylogenetic analysis was performed using BioEdit, ClustalW, and MEGA7 softwares. Results: The clustering of the strains isolated not only from Asia but also from Europe into one common Asian-like group of EIV was observed. Twelve nucleotide substitutions in the M gene of strains from the Asian-like group were crucial for the evolutionary analysis. We also observed homology in the M gene of the Asian-like and H7N7 strains. Conclusions: M gene specific for the Asian-like group is present in strains recently isolated in Europe and Asia, which were classified previously in the Florida 2 clade based on HA. Therefore, Asian-like group does not seem to be assigned to a specific geographical region. Traces of H7N7 strains in more conservative genes like M of some contemporary EIV strains may indicate the link between the old phylogenetic group and recent H3N8 strains. Analysis of conservative genes may be more useful in tracking the direction of virus evolution than in the genes where the high variability rate may blur the original relationships.
机译：介绍：传统上，马流感病毒（EIV）的进化分析基于HA基因。然而，根据分析的基因，流感病毒的特异性使病毒菌株分类为不同的系统发育基团。该研究的目的是基于MA基因分析基于MA基因的EIV的系统发育路径。材料和方法：抛光分离株的M基因已被测序和分析，以及Genbank数据库中可用的所有M序列。使用生物细胞，Clustalw和Mega7软件进行系统发育分析。结果：菌株的聚类不仅来自亚洲，而且从欧洲分为一个普通的亚洲样亚洲EIV群体。来自亚洲样菌株的M个基因的12个核苷酸取代对于进化分析至关重要。我们还观察到亚洲样和H7N7菌株的M基因中的同源性。结论：亚洲群体特异性的M类基因存在于最近欧洲和亚洲孤立的菌株中，以前在佛罗里达州2个基于HA的佛罗里达州2世纪分类。因此，亚洲样组似乎没有被分配给特定的地理区域。在一些当代EIV菌株中的更多保守基因中的H7N7菌株的迹线可以表明旧系统发育基团和最近的H3N8菌株之间的联系。保守基因的分析在跟踪病毒演化方向方面可能比在高可变性率可能模糊原始关系中的基因中更具有用的。

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