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首页> 外文期刊>Journal of Veterinary Research >Leptospira interrogans serogroup Sejroe serovar Hardjo in aborting cows: two herd cases in Sicily (Italy)
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Leptospira interrogans serogroup Sejroe serovar Hardjo in aborting cows: two herd cases in Sicily (Italy)

机译：Leptospira interrogans Serogroup Sejroe Serovar Hardjo中止奶牛：两个畜群案例在西西里岛（意大利）

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摘要

Introduction: The aim of this study was to present two outbreaks of bovine abortion due to Leptospira infection in cattle herds located in the northern part of Sicily (Italy). The animals were positive for Leptospira interrogans serogroup Sejroe serovar Hardjo in a microscopic agglutination test (MAT). Material and Methods: A total of 23 Charolaise cows (farm A) and 75 Limousine bulls and Cinisara and Modicana cows (farm B) were enrolled in this study. The blood samples were collected from all subjects at the following time points: before a cycle of intramuscular treatment with oxytetracycline dihydrate (T0), after 5-6 weeks from the treatment (T1), and every 10 weeks until seronegativisation (T2 in Farm A and T3 in Farm B). A serological test (MAT) was used for the diagnosis of leptospirosis. Results: Two samples from farm A (2/23) and 29 samples from farm B (29/75) were positive to Leptospira interrogans, serogroup Sejroe, serovar Hardjo in the MAT. Leptospira spp. DNA was detected by real-time PCR in the urine sample of one positive cow on farm A, and in placenta and brain samples belonging to one aborted foetus on farm B. Conclusion: It is important to use serological and molecular diagnostic techniques complementarily to identify infected individuals.
机译：介绍：本研究的目的是由于位于西西里岛北部（意大利）的牛群中的Leptospira感染，呈现出两次牛流产的疫苗。在微观凝集试验（MAT）中，动物对leptospira interrogans血清群Serovar serovar serovar serovar serovar serovar hardjo呈阳性。材料和方法：共有23个沙丘奶牛（农场A）和75公牛和蛹和Modicana Cows（Farm B）。从以下时间点的所有受试者中收集血液样品：在从治疗（T1）的5-6周后用催产素二水合物（T0）的肌肉内治疗循环，每10周直至酶可gatisation（农场中的T2和T3在农场B）。血清学检测（MAT）用于诊断钩端螺旋体病。结果：来自农场A（2/23）的两种样品（2/23）和29个来自Farm B（29/75）的样品对Leptospira Interrogans，Serogroup Sejroe，Serovar Hardjo on Mil中的阳性。 Leptospira SPP。通过实时PCR在农场A阳性牛的尿液样品中检测到DNA，并且在农场B中的一个中产胎儿的胎盘和脑样品中检测到。结论：使用互补地互动的血清学和分子诊断技术非常重要受感染的个体。

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