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首页> 外文期刊>Journal of Veterinary Science >An enhanced immunochromatographic strip test using colloidal gold nanoparticle-labeled dual-type N proteins for detection of antibodies to PRRS virus
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An enhanced immunochromatographic strip test using colloidal gold nanoparticle-labeled dual-type N proteins for detection of antibodies to PRRS virus

机译：使用胶体金纳米粒子标记的双型N蛋白的增强的免疫层析条试验，用于检测PRRS病毒的抗体

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摘要

Porcine reproductive and respiratory syndrome (PRRS) is recognized as one of the most important infectious diseases causing serious economic loss in the swine industry worldwide. Due to its increasing genetic diversity, a rapid and accurate diagnosis is critical for PRRS control. The immunochromatographic strip test (ICST) is a rapid and convenient type of immunoassay. In this study, an on-site immunochromatographic assay-based diagnostic method was developed for detection of PRRS virus (PRRSV)-specific antibodies. The method utilized colloidal gold nanoparticle-labeled dual-type nucleocapsid proteins encoded by open reading frame 7. We evaluated 991 field samples from pig farms and 66 serum samples from experimentally PRRSV-inoculated pigs. Based on true PRRSV-specific antibody-positive or -negative sera determined by immunofluorescence assay and IgM enzyme-linked immunosorbent assay (ELISA), the specificity and sensitivity of the ICST were 97.5% and 91.1%, respectively, similar to those of a commercial ELISA (IDEXX PRRS X3 Ab). More importantly, the ICST was completed within 15 min and could detect the PRRSV-specific antibody at an earlier stage of infection (3-7 days) than that of ELISA (7+ days). The results demonstrate that the developed ICST has great potential as an on-farm diagnostic method, providing excellent diagnostic performance in a quick and convenient manner.
机译：猪繁殖和呼吸综合征（PRRS）被认为是全世界猪业中造成严重经济损失的最重要的传染病之一。由于其遗传多样性增加，迅速和准确的诊断对于PRRS控制至关重要。免疫层析杆试验（ICST）是一种快速方便的免疫测定型。在该研究中，开发了基于现场的免疫层析测定的诊断方法，用于检测PRRS病毒（PRRSV）的特异性抗体。该方法利用胶体金纳米颗粒标记的双型核衣壳蛋白，其编码于开放阅读框7.我们评估了来自实验PRRSV接种猪的猪场和66个血清样品的991个田间样本。基于真正的PRRSV特异性抗体阳性或通过免疫荧光测定法测定的抗体血清和IgM酶联免疫吸附测定（ELISA），可分别的ICST的特异性和敏感性分别为97.5％和91.1％，类似于商业人士ELISA（IDEXX PRRS X3 AB）。更重要的是，ICST在15分钟内完成，并且可以在早期感染阶段（3-7天）以比ELISA（7天）的阶段检测PRRSV特异性抗体。结果表明，开发的ICST具有巨大的诊断方法，以快速方便的方式提供出色的诊断性能。

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