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首页> 外文期刊>Journal of Virological Methods >Development and evaluation of an indirect ELISA based on recombinant structural protein VP2 to detect antibodies against duck hepatitis A virus
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Development and evaluation of an indirect ELISA based on recombinant structural protein VP2 to detect antibodies against duck hepatitis A virus

机译：基于重组结构蛋白VP2的间接ELISA检测针对鸭肝炎病毒的抗体的间接ELISA的开发和评价

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摘要

An indirect enzyme-linked immunosorbent assay (I-ELISA) based on the VP2 protein of duck hepatitis A virus type 3 (DHAV-3) was established in this study. The optimal dilutions of antigen, serum and goat anti-duck IgG conjugate were 1:1600 (2.23 mu g/mL), 1:160 and 1:2000, respectively. The optimal blocking buffer was 1% skim milk. The cut-off value for the method was 0.25, and the analytical sensitivity of the method was 1:5120. The results of specific evaluation showed that except for DHAV-1, DHAV-3 antisera did not cross-react with any other common duck-sensitive pathogens, indicating that this method can be used to detect DHAV-3 and DHAV-1 antibodies. The coefficients of variation (CVs) were lower than 10 %. The coincidence rate between the VP2-DHAV-3-ELISA and the neutralization test was 93.3 %. In summary, the I-ELISA method based on VP2 protein has high sensitivity, specificity, and coincidence rate compared with the neutralization test and has advantages in serum monitoring. The I-ELISA method based on VP2 protein provides a simple and rapid method for the detection of anti-DHAV antibodies and the epidemiological monitoring of DHAV.
机译：在本研究中建立了基于鸭甲型肝炎病毒3（DHAV-3）的VP2蛋白的间接酶联免疫吸附测定（I-ELISA）。抗原，血清和山羊抗鸭IgG缀合物的最佳稀释液分别为1：1600（2.23μg/ ml），1：160和1：2000。最佳阻断缓冲液是1％的脱脂乳。该方法的截止值为0.25，该方法的分析敏感性为1：5120。特异性评价结果表明，除DHAV-1外，DHAV-3抗血清没有与任何其他常见的野生动物病原体交叉反应，表明该方法可用于检测DHAV-3和DHAV-1抗体。变异系数（CVS）低于10％。 VP2-DHAV-3-ELISA与中和试验之间的巧合率为93.3％。总之，基于VP2蛋白的I-ELISA方法与中和试验相比具有高灵敏度，特异性和符合率，并在血清监测方面具有优势。基于VP2蛋白的I-ELISA方法为检测抗DHAV抗体以及DHAV的流行病学监测提供了一种简单且快速的方法。

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  • 来源
    《Journal of Virological Methods》 |2020年第1期|共6页
  • 作者

    Lai Yalan; He Ling; Cheng Anchun; Wang Mingshu; Ou Xumin; Wen XingJian; Mao Sai; Sun Di; Jia Renyong; Yang Qiao; Wu Ying; Zhu Dekang; Chen Shun; Liu Mafeng; Zhang Shaqiu; Zhao Xin-Xin; Huang Juan; Gao Qun; Liu Yunya; Yu Yanling; Zhang Ling; Tian Bin; Pan Leichang; Rehman Mujeeb Ur; Chen Xiaoyue;

  • 作者单位

    Sichuan Agr Univ Inst Prevent Vet Med Chengdu 611130 Sichuan Peoples R China;

    Sichuan Agr Univ Inst Prevent Vet Med Chengdu 611130 Sichuan Peoples R China;

    Sichuan Agr Univ Inst Prevent Vet Med Chengdu 611130 Sichuan Peoples R China;

    Sichuan Agr Univ Inst Prevent Vet Med Chengdu 611130 Sichuan Peoples R China;

    Sichuan Agr Univ Inst Prevent Vet Med Chengdu 611130 Sichuan Peoples R China;

    Sichuan Agr Univ Inst Prevent Vet Med Chengdu 611130 Sichuan Peoples R China;

    Sichuan Agr Univ Inst Prevent Vet Med Chengdu 611130 Sichuan Peoples R China;

    Sichuan Agr Univ Inst Prevent Vet Med Chengdu 611130 Sichuan Peoples R China;

    Sichuan Agr Univ Inst Prevent Vet Med Chengdu 611130 Sichuan Peoples R China;

    Sichuan Agr Univ Inst Prevent Vet Med Chengdu 611130 Sichuan Peoples R China;

    Sichuan Agr Univ Inst Prevent Vet Med Chengdu 611130 Sichuan Peoples R China;

    Sichuan Agr Univ Key Lab Anim Dis &

    Human Hlth Sichuan Prov Chengdu 611130 Sichuan Peoples R China;

    Sichuan Agr Univ Inst Prevent Vet Med Chengdu 611130 Sichuan Peoples R China;

    Sichuan Agr Univ Inst Prevent Vet Med Chengdu 611130 Sichuan Peoples R China;

    Sichuan Agr Univ Inst Prevent Vet Med Chengdu 611130 Sichuan Peoples R China;

    Sichuan Agr Univ Inst Prevent Vet Med Chengdu 611130 Sichuan Peoples R China;

    Sichuan Agr Univ Inst Prevent Vet Med Chengdu 611130 Sichuan Peoples R China;

    Sichuan Agr Univ Inst Prevent Vet Med Chengdu 611130 Sichuan Peoples R China;

    Sichuan Agr Univ Inst Prevent Vet Med Chengdu 611130 Sichuan Peoples R China;

    Sichuan Agr Univ Inst Prevent Vet Med Chengdu 611130 Sichuan Peoples R China;

    Sichuan Agr Univ Inst Prevent Vet Med Chengdu 611130 Sichuan Peoples R China;

    Sichuan Agr Univ Inst Prevent Vet Med Chengdu 611130 Sichuan Peoples R China;

    Sichuan Agr Univ Inst Prevent Vet Med Chengdu 611130 Sichuan Peoples R China;

    Sichuan Agr Univ Inst Prevent Vet Med Chengdu 611130 Sichuan Peoples R China;

    Sichuan Agr Univ Inst Prevent Vet Med Chengdu 611130 Sichuan Peoples R China;

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  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 医学微生物学（病原细菌学、病原微生物学）;
  • 关键词

    Duck hepatitis A virus type 3; VP2 protein; Indirect ELISA (I-ELISA); Antibody detection;

    机译：鸭丙型肝炎病毒型3;VP2蛋白质;间接ELISA（I-ELISA）;抗体检测;

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