From fluorescence polarization to Quenchbody: Recent progress in fluorescent reagentless biosensors based on antibody and other binding proteins

UedaH.; DongJ.

首页> 外文期刊>Biochimica et biophysica acta: BBA: International journal of biochemistry, biophysics and molecular biololgy. Proteins and Proteomics >From fluorescence polarization to Quenchbody: Recent progress in fluorescent reagentless biosensors based on antibody and other binding proteins

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From fluorescence polarization to Quenchbody: Recent progress in fluorescent reagentless biosensors based on antibody and other binding proteins

机译：从荧光偏振到猝灭体：基于抗体和其他结合蛋白的无荧光生物传感器的最新进展

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Recently, antibody-based fluorescent biosensors are receiving considerable attention as a suitable biomolecule for diagnostics, namely, homogeneous immunoassay and also as an imaging probe. To date, several strategies for "reagentless biosensors" based on antibodies and natural and engineered binding proteins have been described. In this review, several approaches are introduced including a recently described fluorescent antibody-based biosensor Quenchbody, which works on the principle of fluorescence quenching of attached dye and its antigen-dependent release. The merits and possible demerits of each approach are discussed. This article is part of a Special Issue entitled: Recent advances in molecular engineering of antibody.

机译：近来，基于抗体的荧光生物传感器作为用于诊断的合适生物分子，即均质免疫测定法以及作为成像探针而受到了广泛的关注。迄今为止，已经描述了几种基于抗体以及天然和工程结合蛋白的“无试剂生物传感器”的策略。在这篇综述中，介绍了几种方法，包括最近描述的基于荧光抗体的生物传感器Quenchbody，其基于附着染料的荧光猝灭及其抗原依赖性释放的原理进行工作。讨论了每种方法的优缺点。本文是名为“抗体分子工程的最新进展”的一期特刊的一部分。

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《Biochimica et biophysica acta: BBA: International journal of biochemistry, biophysics and molecular biololgy. Proteins and Proteomics》 |2014年第11期|共9页
作者
UedaH.; DongJ.;
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原文格式 PDF
正文语种 eng
中图分类生物化学;
关键词
DARPin; Fluorescence quenching; In vitro translation; Position-specific labeling; Q-body; Reagentless biosensor;

机译：DARPin;荧光猝灭;体外翻译;位置特异性标记;Q-body;无试剂生物传感器;

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1. From fluorescence polarization to Quenchbody: Recent progress in fluorescent reagentless biosensors based on antibody and other binding proteins [J] . UedaH., DongJ. Biochimica et biophysica acta: BBA: International journal of biochemistry, biophysics and molecular biololgy. Proteins and Proteomics . 2014,第11期

机译：从荧光偏振到猝灭体：基于抗体和其他结合蛋白的无荧光生物传感器的最新进展
2. Reagentless fluorescent biosensors from artificial families of antigen binding proteins [J] . Miranda F.F., Brient-Litzler E., Zidane N., Biosensors & Bioelectronics: The International Journal for the Professional Involved with Research, Technology and Applications of Biosensers and Related Devices . 2011,第10期

机译：来自抗原结合蛋白人工家族的无试剂荧光生物传感器
3. Knowledge-based Design of Reagentless Fluorescent Biosensors from Recombinant Antibodies. [J] . Renard M, Belkadi L, Hugo N, Journal of Molecular Biology . 2002,第2期

机译：基于知识的重组抗体无试剂荧光生物传感器设计。
4. Sugar-sensitive FRET biosensor based on concerted conformational change of binding protein and fluorescent protein [C] . China-Japan-Korea Joint Symposium on Enzyme Engineering . 2004

机译：基于结合蛋白和荧光蛋白协同构象变化的糖敏感性FRET生物传感器
5. Development of monomeric fluorescent proteins and fluorescent protein-based biosensors. [D] . Hoi, Hiofan. 2013

机译：单体荧光蛋白和基于荧光蛋白的生物传感器的开发。
6. Binding and signaling of surface-immobilized reagentless fluorescent biosensors derived from periplasmic binding proteins [O] . Robert M. de Lorimier, Yaji Tian, Homme W. Hellinga 2006

机译：源自周质结合蛋白的表面固定无试剂荧光生物传感器的结合和信号传导
7. Binding and signaling of surface-immobilized reagentless fluorescent biosensors derived from periplasmic binding proteins [O] . de Lorimier, Robert M., Tian, Yaji, Hellinga, Homme W. 2006

机译：源自周质结合蛋白的表面固定无试剂荧光生物传感器的结合和信号传导

From fluorescence polarization to Quenchbody: Recent progress in fluorescent reagentless biosensors based on antibody and other binding proteins

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