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首页> 外文期刊>Journal of cellular biochemistry. >Long Non‐Coding RNA MALAT1 Regulates ZEB1 Expression by Sponging miR‐143‐3p and Promotes Hepatocellular Carcinoma Progression
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Long Non‐Coding RNA MALAT1 Regulates ZEB1 Expression by Sponging miR‐143‐3p and Promotes Hepatocellular Carcinoma Progression

机译：长期非编码RNA MALAT1通过海绵MIR-143-3P调节ZEB1表达，并促进肝细胞癌进展

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摘要

ABSTRACT Hepatocellular carcinoma (HCC) is one of the most common malignancies. Long non‐coding RNAs (lncRNAs) are involved in HCC. This study aimed to explore the effects of lncRNA MALAT1 on HCC development. MALAT1, ZEB1, and miR‐143‐3p in HCC tissues was detected by qRT‐PCR. Effect of MALAT1 on the proliferation and metastasis of HCC cells was estimated by cell‐counting, wound‐healing, and transwell assays. Luciferase reporter assays were used to explore miR‐143‐3p's target, ZEB1. QRT‐PCR and Western blot were employed to determine the effects of MALAT1 or/and miR‐143‐3p on ZEB1 expression. MALAT1 was upregulated in HCC tissues. ZEB1 was a target of miR‐143‐3p. miR‐143‐3p binds with MALAT1, and was regulated by MALAT1. The regulation of MALAT1 on ZEB1 was mediated by miR‐143‐3p. Transfection with siR‐MALAT1 significantly inhibited cell proliferation and invasion, while knockdown of miR‐181a partially reversed these effects. Our findings suggest that MALAT1 may regulate ZEB1 expression by sponging miR‐143‐3p and promotes hepatocellular carcinoma progression. J. Cell. Biochem. 118: 4836–4843, 2017. ? 2017 Wiley Periodicals, Inc.
机译：摘要肝细胞癌（HCC）是最常见的恶性肿瘤之一。长期非编码RNA（LNCRNA）参与HCC。本研究旨在探讨LNCRNA MALAT1对HCC开发的影响。通过QRT-PCR检测HCC组织中的Malat1，Zeb1和MiR-143-3P。 MALAT1对HCC细胞增殖和转移的影响通过细胞计数，伤口愈合和转发测定估计。荧光素酶报告器测定用于探索miR-143-3p的靶，Zeb1。使用QRT-PCR和Western印迹来确定Malat1或/和miR-143-3p对Zeb1表达的影响。马拉特1在HCC组织中上调。 Zeb1是miR-143-3p的目标。 miR-143-3p与马拉特1结合，并由Malat1调节。 MALAT1对Zeb1的调节由miR-143-3p介导。用SIR-MALAT1转染显着抑制细胞增殖和侵袭，而MIR-181A的敲低部分扭转了这些效果。我们的研究结果表明，MALAT1可以通过海绵MIR-143-3P调节ZEB1表达，并促进肝细胞癌进展。 J.Cell。生物学习。 118：4836-4843,2017 2017年Wiley期刊，Inc。

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