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首页> 外文期刊>Journal of cellular biochemistry. >Biochemical and molecular study on interleukin‐1β gene expression and relation of single nucleotide polymorphism in promoter region with Type 2 diabetes mellitus
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Biochemical and molecular study on interleukin‐1β gene expression and relation of single nucleotide polymorphism in promoter region with Type 2 diabetes mellitus

机译：白细胞介素-1β基因表达的生化和分子研究及促进型糖尿病促进区单核苷酸多态性的关系

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Abstract Interleukin‐1β (IL‐1β) assumes a centric role in the regulation of immune and inflammatory responses and thus has been recognized in immune mediated diseases like type 2 diabetes mellitus (T2DM). We aimed to investigate expressed level of IL‐1β and its relation with IL‐1β ?511TC polymorphism in T2DM patients. This study enrolled 80 subjects (50 patients with T2DM and 30 healthy control subjects). Laboratory investigations included fasting (FBG) and 2?h postprandial blood sugar (2?h PBG), HBA1c, lipid profile, and renal function tests. Genotyping of IL‐1β ?511TC (rs16944) SNP assay by real‐time PCR and relative quantitation of IL‐1β gene expression transcript by real‐time PCR. Results: T2DM patients had significantly higher FBG and 2?h PBG, HBA1c, LDLc, TC, TG, systolic, and diastolic BP while lower HDLc compared with control group. IL 1‐ β ?511 TC, CC genotype and C allele were significantly associated with risk of T2DM with odds ratio (OR) 4.73, 95%CI (1.21‐18.39) and OR 2.27, 95%CI (1.72‐4.40), respectively. Moreover, diabetic patients had significantly higher IL 1‐ β gene transcript compared with control group ( P? ?0.001). CC genotype of IL 1‐ β ?511 T C had the highest significant level of IL 1‐ β gene transcript demonstrated compared with C/T and T/T genotypes ( P? ?0.001) in patients. Conclusion: C allele of IL‐1 β ?511 T C could be considered risk factor contributor to T2DM and excess level of IL‐1 β transcript may disclose to some degree the inflammatory role of cytokines in T2DM.
机译：摘要白细胞介素-1β（IL-1β）假设在免疫和炎症反应的调节中具有中心作用，因此已经在免疫介导的疾病中公认，如2型糖尿病（T2DM）。我们旨在调查表达的IL-1β水平及其与IL-1β（IL-1β）的关系，在T2DM患者中的C多态性。本研究注册了80名受试者（50例T2DM和30名健康对照受试者）。实验室调查包括禁食（FBG）和2？H餐后血糖（2？H PBG），HBA1C，脂质分布和肾功能试验。 IL-1βα的基因分型（RS16944）SNP测定通过实时PCR通过实时PCR进行IL-1β基因表达转录物的相对定量。结果：T2DM患者的FBG和2·H PBG，HBA1C，LDLC，TC，TG，收缩型和舒张BP，与对照组更低的HDLC。 IL 1-β？511 T＆ C，CC基因型和C等位基因与T2DM风险显着相关，具有差异比（或）4.73,95％CI（1.21-18.39）和或2.27,95％CI（1.72-4.40） ， 分别。此外，与对照组相比，糖尿病患者具有明显高于IL的IL 1-β基因转录物（p≤1.001）。 IL 1-β的CC基因型？511 T＆GT; C在患者中与C / T和T / T基因型（P = 0.001）相比，具有最高的IL 1-β基因转录物的IL 1-β基因转录物。结论：I IL-1βα的C等位基因511 T＆GT; C可以被认为是T2DM的风险因素因素，并且IL-1β转录物的过量水平可公开于某些程度的细胞因子在T2DM中的作用。

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