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首页> 外文期刊>Journal of cellular biochemistry. >The power of precise bioinformatics prediction of miRNA:mRNA interactions:miR‐4699 as a potential inducer of Wnt signaling pathway
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The power of precise bioinformatics prediction of miRNA:mRNA interactions:miR‐4699 as a potential inducer of Wnt signaling pathway

机译：miRNA的精确生物信息学预测的功率：mRNA相互作用：miR-4699作为Wnt信号通路的潜在诱导剂

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Abstract microRNAs have attracted interest because of their regulatory effects on gene expression. Experimental detection of potential targets of miRNAs is a laborious task. Considering the expensive techniques of detection, computational approaches for miRNA target prediction can be used as the first step in miRNA research. A large number of tools and algorithms have been developed during the last two decades, led to problems such as confusion in selecting an appropriate tool and false positive or negative results. Therefore, one of the most frequent problems and critical issues of miRNA research is finding a reliable miRNA target prediction tool. In this study, we have proposed a research direction and introduced user‐friendly and current databases and tools with the highest accuracy. To verify whether our proposed research direction is practical, we have provided a case example of predicting a miRNA which can target negative regulators of osteogenesis and experimentally evaluated the accuracy of the prediction results by Real‐Time PCR and Luciferase assay. The results of RT‐qPCR and Luciferase assay indicated a significant decline in expression of Dickkopf‐related protein 1 ( DKK1 ) and tumor necrosis factor ligand superfamily member 11 ( TNFSF11 ) as the key negative regulators of osteogenesis upon overexpression of miR‐4699‐3p. The results emphasize the validity and importance of accurate in silico investigation as the first step in experimental studies. This is the first report detailing the prediction and validation of miR‐4699‐3p target genes. We suggest hsa‐miR‐4699 for further investigation as an osteogenic miRNA for therapeutics purposes.
机译：摘要MicroRNAS由于对基因表达的调节作用而引起了兴趣。 MiRNA潜在目标的实验检测是一个艰苦的任务。考虑到昂贵的检测技术，MiRNA靶预测的计算方法可以用作miRNA研究的第一步。在过去二十年中已经开发了大量工具和算法，导致了选择适当的工具和假阳性或负面结果等困惑。因此，miRNA研究中最常见的问题和关键问题是找到可靠的miRNA目标预测工具。在这项研究中，我们提出了一种研究方向，并引入了具有最高精度的用户友好和当前的数据库和工具。为了验证我们提出的研究方向是否实用，我们已经提供了预测能够靶向成骨的阴性调节剂的miRNA的案例的例子，并通过实时PCR和荧​​光素酶测定进行实验评估预测结果的准确性。 RT-QPCR和荧​​光素酶测定结果表明Dickkopf相关蛋白1（DKK1）和肿瘤坏死因子配体超细成员11（TNFSF11）表达的显着下降，因为骨骨关键发生在miR-4699-3p时的关键负调节剂。结果强调了硅调查中准确的有效性和重要性作为实验研究的第一步。这是第一个报告详细说明miR-4699-3p靶基因的预测和验证。我们建议HSA-MIR-4699进行进一步调查作为治疗性目的的骨质原骨髓内甲醛。

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