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首页> 外文期刊>Journal of cellular biochemistry. >Microporous cellulosic scaffold as a spheroid culture system modulates chemotherapeutic responses and stemness in hepatocellular carcinoma
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Microporous cellulosic scaffold as a spheroid culture system modulates chemotherapeutic responses and stemness in hepatocellular carcinoma

机译：作为球状培养系统的微孔纤维素支架调节肝细胞癌的化疗反应和茎秆

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摘要

Hepatocellular carcinoma (HCC) treatments are evaluated by two-dimensional (2D) in vitro culture systems, despite their limited ability to predict drug efficacy. The three-dimensional (3D) microporous scaffold provides the possibility of generating more reliable preclinical models to increase the efficacy of cancer treatments. The physical properties of a microporous cellulosic scaffold were evaluated. The cellulosic scaffold was biocompatible and had a highly porous network with appropriate pore size, swelling rate, and stiffness of cancer cell cultures. Cellulosic scaffolds were compared with 2D polystyrene for the culture of HepG2 and Huh7 human HCC cells. Cellulosic scaffolds promoted tumor spheroid formation. Cells cultured on scaffolds were more resistant to chemotherapy drugs and showed upregulation of EpCAM and Oct4. The migration ability of HCC cells cultured on scaffolds was significantly greater than that of cells grown in 2D cultures as evidenced by the downregulation of E-cadherin. In addition, the proportion of CD44+/CD133+ HCC cancer stem cells (CSCs) was significantly greater in cells cultured on scaffolds than in those grown in 2D cultures. These findings suggest that cellulosic scaffolds effectively mimic the in vivo tumor behavior and may serve as a platform for the study of anticancer therapeutics and liver CSCs.
机译：尽管预测药物功效的能力有限，但肝细胞癌（2D）在体外培养系统中评估了肝细胞癌（2D）治疗。三维（3D）微孔脚手架提供了产生更可靠的临床前模型以增加癌症处理的疗效。评价微孔纤维素支架的物理性质。纤维素支架是生物相容性的，具有高度多孔的网络，具有适当的孔径，溶胀率和癌细胞培养物的刚度。将纤维素支架与2D聚苯乙烯进行比较，用于HEPG2和HUH7人HCC细胞的培养。纤维素支架促进肿瘤球状形成。在支架上培养的细胞对化疗药物更耐药，并显示出EPCAM和OCT4的上调。在支架上培养的HCC细胞的迁移能力显着大于E-Cadherin的下调所证明的2D培养物中的细胞的迁移能力。此外，CD44 + / CD133 + HCC癌症干细胞（CSCs）的比例在支架上培养的细胞中显着大于在2D培养物中生长的细胞中。这些发现表明，纤维素支架有效地模仿体内肿瘤行为，可以作为研究抗癌治疗和肝脏CSC的平台。

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