...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>魚病研究 >Properties of viral hemorrhagic septicemia virus (VHSV) isolated from Japanese flounder Paralichthys olivaceus
【24h】

Properties of viral hemorrhagic septicemia virus (VHSV) isolated from Japanese flounder Paralichthys olivaceus

机译：病毒出血性杂草病毒（VHSV）的性质从日本比目鱼Paralichthys Olivaceus孤立

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Physiological properties and pathogenicity of three isolates of viral hemorrhagic sepicemia virus (VHSV), Obama25 isolated from wild Japanese flounder Paralichthys olivaceus in 1999, JF00Ehi1 from farmed diseased Japanese flounder in 2000 and KRRV9601 from farmed diseased Japanese flounder in 1996, were studied. The former two isolates belong to American genotype (genogroup 1) and the latter one to European genotype (genogroup 111). Among three fish cell lines tested, the three isolates multiplied best in FHM cells, moderately in EPC cells but hardly in RTG-2 cells. In FHM cells, these isolates multiplied at 10, 15, and 20degreesC but not at 25degreesC. The optimum temperature was 15degreesC for KRRV9601 and 20degreesC for the other two isolates. In in vitro stability tests in different waters at different temperatures, the viral infectivity decreased rapidly in non-treated seawater with increasing water temperature. The two American genotype isolates, Obama25 and JIF00Ehi1, exhibited a similar level of virulence in flounder at 13degreesC causing disease signs similar to those observed in naturally affected flounder. On the other hand, no mortality was produced by KRRV9601, though the virus was re-isolated from surviving fish. These results in the pathogenicity test support the fact that the American genotype of VHSV has been prevailing among wild and farmed flounder in Japan.
机译：1999年1999年，从野生日本比尔巴拉哌猴菌（VHSV），奥巴马25分离出的病毒出血性肠脓毒症病毒（VHSV）的生理特性和致病性。从1996年的农业患者日本比赛中的JF00HI1和1996年来自农业疫苗日本比赛的KRRV9601。前两种分离株属于美国基因型（Genogroup 1）和后一种对欧洲基因型（GenoGroup 111）。在测试的三种鱼细胞系中，三个分离株在FHM细胞中倍增，适度在EPC细胞中，但在RTG-2细胞中几乎没有。在FHM细胞中，这些隔离物乘以10,15和20degreesc但不在25degreesc。对于其他两个分离株，最佳温度为15dgregReesc为krrv9601和20degreesc。在不同温度下的不同水处的体外稳定性试验中，在未处理的海水中随着水温的未处理海水中的病毒感染速度迅速下降。两种美国基因型分离物，奥巴马25和Jif00ehi1在13℃的比目鱼中表现出类似的毒力水平，导致疾病标志类似于在天然受影响的比目鱼中观察到的疾病。另一方面，KRRV9601不会产生死亡率，尽管病毒从幸存的鱼中重新分离出病毒。这些导致致病性测试支持，VHSV的美国基因型在日本的野生和农业比赛中一直在普遍存在。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号