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首页> 外文期刊>Biotechnology and Bioengineering >Encapsulation of proteins in biodegradable polymeric microparticles using electrospray in the Taylor Cone-Jet mode
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Encapsulation of proteins in biodegradable polymeric microparticles using electrospray in the Taylor Cone-Jet mode

机译：在泰勒锥喷射模式下使用电喷雾将蛋白质包封在可生物降解的聚合物微粒中

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摘要

Solvent extraction (or evaporation from a W-1/O/W-2-dispersion), coacervation, and spray drying methods are commonly employed to encapsulate protein drugs in polymeric microparticles for sustained delivery applications. To overcome the limitations of these methods, a novel electrospray method was developed to encapsulate a model protein drug-bovine serum albumin (BSA) in biodegradable polymeric microparticles and examine the feasibility of the process in not denaturing the protein. Microparticles of approximately 20 mu m diameter with corrugated surfaces and smooth surfaces were observed by scanning electron microscope. Confocal laser scanning microscope images showed that BSA was distributed evenly in microparticles. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDSPAGE) was employed to investigate the protein integrity of BSA released from the polymer matrix after 38 days. No protein degradation was observed during the 38 days release. The secondary structure of released BSA was characterized by Fourier transform infrared (FTIR) and circular dichroism (CD), which suggested that the released BSA was almost identical to native BSA. The encapsulation efficiency could reach 76% by adjusting the amount of the additive Pluronic F127 and processing parameters. The release profile could be tailored by the fabrication process and the sustained release of BSA could endure for more than 1 month. More than 80% of the bioactivity of BSA (evaluated by BSA ELISA kit) could be maintained after releasing from polymer matrix. Findings of the present study demonstrate that this novel electrospray method is a promising approach to encapsulate bioactive materials such as proteins, enzymes, antibiotics, and DNA fragments in biodegradable polymeric particles.
机译：溶剂提取（或从W-1 / O / W-2-分散体蒸发），凝聚和喷雾干燥方法通常用于将蛋白质药物包裹在聚合物微粒中，以实现持续递送应用。为了克服这些方法的局限性，开发了一种新颖的电喷雾方法，将模型蛋白药物-牛血清白蛋白（BSA）封装在可生物降解的聚合物微粒中，并检验了该方法不使蛋白变性的可行性。通过扫描电子显微镜观察到直径约20μm的具有波纹表面和光滑表面的微粒。共聚焦激光扫描显微镜图像显示，BSA均匀分布在微粒中。十二烷基硫酸钠-聚丙烯酰胺凝胶电泳（SDSPAGE）用于研究38天后从聚合物基质中释放的BSA的蛋白质完整性。在38天释放期间未观察到蛋白质降解。释放的BSA的二级结构的特征在于傅立叶变换红外（FTIR）和圆二色性（CD），这表明释放的BSA与天然BSA几乎相同。通过调整添加剂Pluronic F127的数量和加工参数，封装效率可以达到76％。释放曲线可以根据制造工艺进行调整，BSA的持续释放可以持续1个月以上。从聚合物基质中释放出来后，可以维持80％以上的BSA生物活性（通过BSA ELISA试剂盒评估）。本研究的发现表明，这种新颖的电喷雾方法是一种在生物可降解的聚合物颗粒中包裹蛋白质，酶，抗生素和DNA片段等生物活性材料的有前途的方法。

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