Histochemical detection of laccase in Pycnoporus cinnabarinus using microwave-enhanced colloidal gold microcrystallization

Cameron L. Jones; Greg T. Lonergan

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Histochemical detection of laccase in Pycnoporus cinnabarinus using microwave-enhanced colloidal gold microcrystallization

机译：微波胶体金微晶法检测朱砂比目鱼漆酶的组织化学

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Laccase was detected histochemically in Pycnoporus cinnabarinus fungal hyphae by growing the organism on cellophane or microporous polycarbonate membranes overlaid on malt extract agar. Membrane sections were stained with a reagent of 2,6-dimethoxyphenol and colloidal gold for 30 min and irradiated for 24 s at maximum output in a microwave oven. Laccase was detected as purple-blue deposits at the apical tip region in high concentrations, and at lower levels on the exterior, sub-apical hyphal sheath.

机译：通过在玻璃纸或覆盖在麦芽提取物琼脂上的微孔聚碳酸酯膜上生长该生物，可以在朱红比索菌真菌菌丝中化学检测到漆酶。将膜切片用2,6-二甲氧基苯酚和胶体金试剂染色30分钟，并在微波炉中以最大输出量照射24 s。漆酶以高浓度在紫红色的沉淀物被检测到在紫色的顶端区域，而在外部的，低于顶端的菌丝鞘中含量较低。

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《Biotechnology Techniques》 |1999年第12期|共5页
作者
Cameron L. Jones; Greg T. Lonergan;
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原文格式 PDF
正文语种 eng
中图分类分子生物学;
关键词
fungla enzymes; histochemistry; laccase; microwave radiation; Pycnoporus cinnabarinus;

机译：真菌酶;组织化学;漆酶;微波辐射;朱砂比目鱼;

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专利

1. Histochemical detection of laccase in Pycnoporus cinnabarinus using microwave-enhanced colloidal gold microcrystallization [J] . Cameron L. Jones, Greg T. Lonergan Biotechnology Techniques . 1999,第12期

机译：微波胶体金微晶法检测朱砂比目鱼漆酶的组织化学
2. DAIRY WASTE ‘GHEE RESIDUE’ INFLUENCE LACCASES PRODUCTION BY PYCNOPORUS CINNABARINUS AND ITS OPTIMIZATION [J] . Rasheeda Khanam, Dr. R.Gyana Prasuna International Journal of Applied Biology and Pharmaceutical Technology . 2013,第4期

机译：肉桂芥（Pycnoporus cinnabarinus）生产乳制品废物“酥油渣油”的影响酶及其优化
3. Laccase from Pycnoporus cinnabarinus and phenolic compounds: Can the efficiency of an enzyme mediator for delignifying kenaf pulp be predicted? [J] . Andreu G., Vidal T. Bioresource Technology: Biomass, Bioenergy, Biowastes, Conversion Technologies, Biotransformations, Production Technologies . 2013,第Null期

机译：朱砂比丘比尔（Pycnoporus cinnabarinus）和酚类化合物的漆酶：能否预测酶介导的洋麻浆脱木质素的效率？
4. Bioinformatics Analysis of Laccase (Lac1) in Pycnoporus cinnabarinus [C] . Li He, Zhou Guoying, Zhang Huaiyun, IITA International Conference on Nanotechnology and Computer Engineering . 2010

机译：Pycnoporus incnabarinus的漆酶（LAC1）的生物信息分析
5. Pycnoporus cinnabarinus : extraction, isolation et caractérisation de molécules ayant un effet cytotoxique [D] . Fortin, Sophie. 2018

机译：Pycnoporus cinnnabarinus：具有细胞毒性作用的分子的提取，绝缘和表征
6. Engineering Platforms for Directed Evolution of Laccase from Pycnoporus cinnabarinus [O] . S. Camarero, I. Pardo, A. I. Cañas, 2012

机译：朱砂比目鱼漆酶定向进化的工程平台
7. Engineering platforms for directed evolution of Laccase from Pycnoporus cinnabarinus [O] . Camarero, S., Pardo, I., Cañas, A. I., 2012

机译：用于从朱砂比目鱼（Pycnoporus cinnabarinus）的漆酶定向进化的工程平台

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Histochemical detection of laccase in Pycnoporus cinnabarinus using microwave-enhanced colloidal gold microcrystallization

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