...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Biotechnology Advances: An International Review Journal >Contemporary molecular tools in microbial ecology and their application to advancing biotechnology
【24h】

Contemporary molecular tools in microbial ecology and their application to advancing biotechnology

机译：微生物生态学中的当代分子工具及其在生物技术发展中的应用

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Novel methods in microbial ecology are revolutionizing our understanding of the structure and function of microbes in the environment, but concomitant advances in applications of these tools to biotechnology are mostly lagging behind. After more than,a century of efforts to improve microbial culturing techniques, about 70-80% of microbial diversity - recently called the "microbial dark matter" - remains uncultured. In early attempts to identify and sample these so far uncultured taxonomic lineages, methods that amplify and sequence ribosomal RNA genes were extensively used. Recent developments in cell separation techniques, DNA amplification, and high-throughput DNA sequencing platforms have now made the discovery of genes/genomes of uncultured microorganisms from different environments possible through the use of metagenomic techniques and single-cell genomics. When used synergistically, these metagenomic and single-cell techniques create a powerful tool to study microbial diversity. These genomics techniques have already been successfully exploited to identify sources for i) novel enzymes or natural products for biotechnology applications, ii) novel genes from extremophiles, and iii) whole genomes or operons from uncultured microbes. More can be done to utilize these tools more efficiently in biotechnology. (C) 2015 Elsevier Inc. All rights reserved.
机译：微生物生态学中的新方法正在彻底改变我们对环境中微生物结构和功能的理解，但这些工具在生物技术中的应用进展却相对滞后。经过一个多世纪的努力，改善了微生物培养技术，大约70-80％的微生物多样性-最近被称为“微生物暗物质”-仍未培养。在早期鉴定和取样这些迄今未培养的生物分类谱系的尝试中，广泛使用了扩增和测序核糖体RNA基因的方法。细胞分离技术，DNA扩增和高通量DNA测序平台的最新发展现已使通过使用宏基因组学技术和单细胞基因组学发现来自不同环境的未培养微生物的基因/基因组成为可能。当协同使用时，这些宏基因组学和单细胞技术创建了一个强大的工具来研究微生物多样性。这些基因组学技术已经成功地用于鉴定以下方面的资源：i）用于生物技术应用的新型酶或天然产物，ii）来自极端微生物的新基因，以及iii）来自未培养微生物的整个基因组或操纵子。要在生物技术中更有效地利用这些工具，还可以做更多的工作。 （C）2015 Elsevier Inc.保留所有权利。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号