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首页> 外文期刊>Biotechnology Advances: An International Review Journal >Synthesis, regulation and production of urokinase using mammalian cell culture: a comprehensive review.
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Synthesis, regulation and production of urokinase using mammalian cell culture: a comprehensive review.

机译：利用哺乳动物细胞培养合成，调节和生产尿激酶：全面综述。

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摘要

Urokinase, a serine protease, catalyzes the conversion of plasminogen to plasmin, which is responsible for dissolution of clots in blood vessels. It is an important drug for treatment of thromboembolic disease. Production of urokinase by mammalian cell culture has the following important steps: synthesis, regulation and secretion. Production and accumulation of this product in a bioreactor is a real challenge for biochemical engineers. Considerable information at molecular level needs to be understood for production of urokinase in order to correlate different parameters, which in turn can maximize the productivity. This information will be highlighted in this review. Moreover, urokinase production is a product-inhibited process. Therefore, in situ urokinase separation strategy is required to operate a bioreactor at its maximum urokinase formation rate. Integrated urokinase production and isolation processes developed recently will also be discussed briefly in this review.
机译：尿激酶是一种丝氨酸蛋白酶，催化纤溶酶原向纤溶酶的转化，纤溶酶负责血管内血块的溶解。它是治疗血栓栓塞性疾病的重要药物。通过哺乳动物细胞培养产生尿激酶有以下重要步骤：合成，调节和分泌。对于生物化学工程师来说，在生物反应器中生产和积累这种产品是一个真正的挑战。尿激酶的产生需要在分子水平上理解大量信息，以便关联不同的参数，从而可以使生产率最大化。此信息将在此评论中突出显示。此外，尿激酶的产生是产物抑制过程。因此，需要原位尿激酶分离策略以最大的尿激酶形成速率操作生物反应器。最近开发的综合尿激酶生产和分离方法也将在本综述中进行简要讨论。

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