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首页> 外文期刊>Biotechnology Advances: An International Review Journal >Intelligent bioprocessing for haemotopoietic cell cultures using monitoring and design of experiments
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Intelligent bioprocessing for haemotopoietic cell cultures using monitoring and design of experiments

机译：使用监测和实验设计对造血细胞培养进行智能生物处理

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摘要

The need for successful ex-vivo expansion and directed differentiation of haematopoietic stem cells (HSCs) for therapeutic applications has increased over the past decade. Haematopoietic cell cultures are complex and full characterisation of the process environment has yet to be achieved. The complexity and transient nature of HSC cultures make the identification, maintenance and control of optimal operating conditions challenging. Application of real-time, on-line monitoring techniques and process control strategies enhances the ability to operate bioprocesses of desired reproducibility and high product quality. In this review, we discussed the methods by which in vitro culture information necessary for bioprocess control may be obtained, including process considerations, monitoring and analytical tools, and design of experiments (DOE). The successful application of these tools may result in time- and cost-effective cultures for directed differentiation and expansion of haematopoietic components intended for clinical use.
机译：在过去的十年中，对用于治疗应用的造血干细胞（HSC）的成功的体外扩增和定向分化的需求不断增长。造血细胞培养非常复杂，尚未完全表征过程环境。 HSC培养物的复杂性和短暂性使识别，维护和控制最佳操作条件变得颇具挑战性。实时在线监测技术和过程控制策略的应用增强了操作具有所需可重复性和高产品质量的生物过程的能力。在这篇综述中，我们讨论了获得生物过程控制所需的体外培养信息的方法，包括过程考虑因素，监测和分析工具以及实验设计（DOE）。这些工具的成功应用可能会导致节省时间和成本效益的培养，用于定向分化和扩大打算用于临床的造血成分。

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