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首页> 外文期刊>Biotechnology Advances: An International Review Journal >Biotechnological advances in mango (Mangifera indica L.) and their future implication in crop improvement - A review
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Biotechnological advances in mango (Mangifera indica L.) and their future implication in crop improvement - A review

机译:芒果(Mangifera indica L.)的生物技术进步及其对作物改良的未来意义-评论

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摘要

Biotechnology can complement conventional breeding and expedite the mango improvement programmes. Studies involving in vitro culture and selection, micropropagation, embryo rescue, genetic transformation, marker-assisted characterization and DNA fingerprinting, etc. are underway at different centers worldwide. In vitro culture and somatic embryogenesis of several different genotypes have been achieved. The nucellus excised from immature fruitlets is the appropriate explant for induction of embryogenic cultures. High frequency somatic embryogenesis has been achieved in some genotypes; however, some abnormalities can occur during somatic embryo germination. Embryo rescue from young and dropped fruitlets can improve the hybridization success in a limited flowering season. Protocols for protoplast culture and regeneration have also been developed. In vitro selections for antibiotic tolerance and fungal toxin resistance have been very promising for germplasm screening. Genetic transformation using Agrobacterium tumefaciens has been reported. Genes that are involved with fruit ripening have been cloned and there have been attempts to deliver these genes into plants. DNA fingerprinting and studies on genetic diversity of mango cultivars and Mangifera species are also being conducted at several research stations. The purpose of this review is to focus upon contemporary information on biotechnological advances made in mango. It also describes some ways of overcoming the problems encountered during in vitro propagation of mango.
机译:生物技术可以补充常规育种并加快芒果改良计划。世界各地的不同中心正在进行有关体外培养和选择,微繁殖,胚胎拯救,遗传转化,标记辅助表征和DNA指纹图谱等的研究。已经实现了几种不同基因型的体外培养和体细胞胚胎发生。从未成熟小果中切除的细胞核是诱导胚胎发生培养物的合适外植体。在某些基因型中已经实现了高频体细胞胚发生。但是,在体细胞胚萌发过程中可能会发生一些异常。从幼小和掉落的小果实中拯救胚胎可以在有限的开花季节提高杂交的成功率。还已经开发了原生质体培养和再生的方案。对于种质筛选,抗生素耐受性和真菌毒素抗性的体外选择非常有前途。已经报道了使用根癌农杆菌的遗传转化。已经克隆了与果实成熟有关的基因,并且已经尝试将这些基因传递到植物中。还在几个研究站进行了DNA指纹图谱以及芒果品种和Mangifera物种遗传多样性的研究。这篇综述的目的是集中于有关芒果生物技术进步的当代信息。它还描述了克服芒果体外繁殖过程中遇到的问题的一些方法。

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