Antioxidants increase gene transfection efficiency of electroporation in Blymphoma cells

Liang JF.; Yoon YG.; Kim SC.

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Antioxidants increase gene transfection efficiency of electroporation in Blymphoma cells

机译：抗氧化剂可提高淋巴瘤细胞电穿孔基因的转染效率

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Treatments of B lymphoma cells with dimethyl sulfoxide, nitric oxide, N-acetylcysteine, or pencillamine increased the gene transfection efficiency of electroporation (similar to 8-fold) without affecting cell viability. Decrease of electro-induced oxidative stress may explain antioxidant-increased electroporation efficiency.

机译：用二甲基亚砜，一氧化氮，N-乙酰基半胱氨酸或pennylamine处理B淋巴瘤细胞可提高电穿孔的基因转染效率（类似于8倍），而不会影响细胞活力。电诱导的氧化应激的降低可以解释抗氧化剂提高的电穿孔效率。

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《Biotechnology Techniques》 |1998年第8期|共4页
作者
Liang JF.; Yoon YG.; Kim SC.;
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正文语种 eng
中图分类分子生物学;
关键词
Epstein-barr-virus; Dimethyl-sulfoxide; Activation; Apoptosis;

机译：EB病毒;二甲基亚砜;活化;凋亡;

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专利

1. Antioxidants increase gene transfection efficiency of electroporation in Blymphoma cells [J] . Liang JF., Yoon YG., Kim SC. Biotechnology Techniques . 1998,第8期

机译：抗氧化剂可提高淋巴瘤细胞电穿孔基因的转染效率
2. Mild hyperthermia prior to electroporation increases transfection efficiency in HCT 116, HeLa S3 and SGC 7901 cells [J] . Zheng-Li Wei, Ryohei Ogawa, Ichiro Takasaki, Biotechnology Letters . 2010,第3期

机译：电穿孔前的轻度高温会增加HCT 116，HeLa S3和SGC 7901细胞的转染效率
3. High efficiency transient transfection of genes in human umbilical vein endothelial cells by electroporation. [J] . Ear T, Giguere P, Fleury A, Journal of Immunological Methods . 2001,第1a2期

机译：通过电穿孔高效瞬时转染人脐静脉内皮细胞中的基因。
4. A purely electronic method to measure transfection efficiency in a single-cell electroporation biochip [C] . G. Cellere, L. Bandiera, M. Borgo, Meeting of the Electrochemical Society . 2008

机译：一种纯电子方法，用于测量单细胞电穿孔生物芯片中的转染效率
5. Microscale Electroporation for Transfection of Genetic Constructs into Adherent Secondary Cells and Primary Neurons in Culture [D] . Patel, Chetan. 2012

机译：微型电穿孔法将遗传构建体转染到培养中的粘附次级细胞和原代神经元中
6. Enhanced transfection efficiency and improved cell survival after electroporation of G2/M-synchronized cells and treatment with sodium butyrate. [O] . S Goldstein, C M Fordis, B H Howard 1989

机译：G2 / M同步细胞电穿孔并用丁酸钠处理后可提高转染效率并改善细胞存活率。
7. Nonviral transfection of distinct types of human dendritic cells: high-efficiency gene transfer by electroporation into hematopoietic progenitor- but not monocyte-derived dendritic cells [O] . Tendeloo, Van, V.F.I., Snoeck, H.-W., Lardon, F., 1998

机译：不同类型人类树突状细胞的非病毒转染：通过电穿孔将基因高效转移到造血祖细胞而非单核细胞衍生的树突状细胞中

Antioxidants increase gene transfection efficiency of electroporation in Blymphoma cells

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