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首页> 外文期刊>水産增殖 >Gonad formation and pre-meiotic proliferation of gonocytes in the Japanese willow minnow Gnathopogon caerulescens
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Gonad formation and pre-meiotic proliferation of gonocytes in the Japanese willow minnow Gnathopogon caerulescens

机译：在日本柳树内Nnathopogon Caerulescens中的Gonad形成和晚尾增殖

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摘要

This study histologically examined gonadal formation and sex differentiation in the Japanese willow minnow Gnathopogon caerulescens. Larvae and juveniles reared at 20。C were collected in batches at different timepoints [every 5 d from 0 to 20 d post-hatching (dph) and every 10 d from 30 to 50 dph]. In newly hatched larvae, primordial germ cells (PGCs) had migrated to the gonadal anlagen near the dorsal hepatopancreas under the peritoneum, before genital ridge development. At 5 dph, stromal cells accumulated near PGCs, and by15 dph, they proliferated around the PGCs and formed gonadal primordia. The germ cell number remained constant in all fish at 15-20 cells until20 dph; thereafter, in approximately 50% of them, active gonocyte proliferation commenced and underwent meiosis. By 40 dph, the germ cell number in some fish increased beyond 1200， and by 50 dph, ovarian cavities were evident. The remaining fish that did not undergo these differentiation steps developed sperm duct structures at approximately 30 dph and maintained 60-80 germ cells up to 40 dph.
机译：该研究在日本柳树脑病肠杆菌囊腔中组织学检查的Gonadal形成和性别分化。在20.C的幼虫和幼羽在不同的时间点（每5d从0到20d）批量收集，从0至20d过阴影后（DPH），每10d为30至50 dph]。在新孵出的幼虫中，原始生殖细胞（PGCs）迁移到腹膜发育前腹膜下腹膜下背肝病附近的性腺anlagen。在5Pph，在PGCs附近积聚的基质细胞，并达到15pph，它们在PGCS周围增殖并形成了对淋足的原序。在15-20个细胞的所有鱼中，生殖细胞数仍然是恒定的，直到20pph;此后，在其中大约50％的50％中，活性淋病卵细胞增殖开始并经过减数分裂。 ×40％点评，一些鱼中的生殖细胞数量超过1200，并且左四，卵巢腔显而易见。没有经过这些分化步骤的剩余鱼类在大约30分钟内开发出精子管结构，并保持60-80个生殖细胞，高达40％点DPH。

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