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首页> 外文期刊>水産增殖 >Application of spectrophotometry to estimate the optimum culture conditions for Nannochloropsis oculata as a diet for zooplankton
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Application of spectrophotometry to estimate the optimum culture conditions for Nannochloropsis oculata as a diet for zooplankton

机译：分光光度法估算南氯化物Oculata作为浮游动物饮食的最佳培养条件

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摘要

Counting cells is a difficult way to estimate the optimum culture conditions for Nannochloropsis oculata as a feed for rotifers efficiently. To devise a simple tool that could be used quickly to estimate the optimum harvest time using spectrophotometry, we investigated the relationship between N. oculata growth population patterns, absorption spectra and nutrient salt concentrations. The cell densities, absorption values at each wavelength (360-780 nm) and concentrations of nitrate-nitrogen and phosphate were measured daily. The logarithmic phase was terminated just before phosphate limitation. The absorption ratio of 490 nm per 680 nm increased until the start of the logarithmic phase, continued to decrease beyond the logarithmic phase and then increased again just after phosphate starvation. The cells reached a stationary phase after additional nitrate-nitrogen starvation. These results suggest that absorption spectra change the trend gradually in response to nutrient salt starvation rather than to growth phase transition. The present study suggests, the possibility of applying spectrophotometry to monitor N. oculata culture conditions more accurately than the cell count method allows.
机译：计数细胞是估计Nannochloropsis oculata作为有效转子的进料的最佳培养条件的困难方法。为了设计一个可以快速使用的简单工具来估计使用分光光度法来估计最佳的收获时间，我们研究了N.Oculata生长群体模式，吸收光谱和营养浓度之间的关系。每天测量每波长（360-780nm）的蓄积值和硝酸氮和磷酸浓度的细胞密度。在磷酸盐限制之前，对数相终止。每680nm的吸收比率增加，直到对数相开始，继续超出对数相的降低，然后在磷酸盐饥饿后再次增加。在另外的硝酸盐 - 氮饥饿后，细胞达到固定相。这些结果表明，吸收光谱响应营养盐饥饿而不是生长相转变而逐渐改变趋势。本研究表明，施用分光光度法的可能性比细胞计数方法更准确地施加分光光度法以监测N. Oculata培养条件。

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