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首页> 外文期刊>American journal of primatology >A PCR-Based Marker to Simply Identify Saimiri sciureus and S. boliviensis boliviensis
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A PCR-Based Marker to Simply Identify Saimiri sciureus and S. boliviensis boliviensis

机译:基于PCR的标记可以简单地鉴定Saiiri sciureus和S. boliviensis boliviensis

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摘要

Squirrel monkeys, mainly Saimiri sciureus and S. boliviensis, are common in zoos and widely used in biomedical research. However, an exact species identification based on morphological characteristics is difficult. Hence, several molecular methods were proposed, but all of them are expensive and require extensive laboratory work. In contrast, we describe an Alu integration, which is present in S. boliviensis boliviensis and absent in S. sciureus. Among analyzed S. b. peruviensis specimens various presence/absence patterns of the integration were detected indicating that this study population might have originated from a natural hybrid zone. Based on the size of the Alu element (similar to 300 bp), the presence/absence pattern of the integration can easily be traced by PCR and followed by agarose gel electrophoresis. Am. J. Primatol. 70:1177-1180, 2008. (C) 2008 Wiley-Liss, Inc.
机译:松鼠猴,主要是赛蒙(Saimiri sciureus)和玻利维亚链球菌(S. boliviensis),在动物园中很常见,并广泛用于生物医学研究。然而,基于形态特征的精确物种识别是困难的。因此,提出了几种分子方法,但是所有这些方法都很昂贵并且需要大量的实验室工作。相反,我们描述了Alu整合,它存在于boliviensis boliviensis中,而在ssureureus中不存在。在分析的人中。 peruviensis标本检测到整合的各种存在/不存在模式,表明该研究人群可能起源于自然杂种区。根据Alu元件的大小(大约300 bp),可以通过PCR轻松地跟踪整合的存在/不存在模式,然后进行琼脂糖凝胶电泳。上午。 J. Primatol。 70:1177-1180,2008.(C)2008 Wiley-Liss,Inc.

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