...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Allergy >Carbohydrate-based particles reduce allergic inflammation in a mouse model for cat allergy.
【24h】

Carbohydrate-based particles reduce allergic inflammation in a mouse model for cat allergy.

机译：基于碳水化合物的颗粒可减轻小鼠对猫过敏的过敏性炎症。

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

BACKGROUND: Allergen-specific immunotherapy (ASIT) is the only treatment of allergic disease that gives long-lasting relief of symptoms. However, concerns for safety and efficiency have highlighted the need for improvement of the therapy. We have previously suggested carbohydrate-based particles (CBPs) as a novel adjuvant and allergen carrier for ASIT. Our aim of this study was to evaluate the therapeutic potential of CBPs in ASIT, employing a mouse model for cat allergy. METHODS: BALB/c mice were subcutaneously immunized with the recombinant (r) cat allergen Fel d 1 followed by intranasal challenge with cat dander extract (CDE). The sensitized mice were therapeutically treated with rFel d 1 covalently coupled to CBPs (CBP-rFel d 1). Airway hyper-reactivity (AHR), infiltration of leucocytes in bronchoalveolar lavage (BAL) fluid, allergen-specific serum immunoglobulin levels and in vitro splenocyte responses were evaluated. RESULTS: Mice treated with CBP-rFel d 1 showed reduced features of allergic inflammation. They responded with (i) significantly decreased AHR and infiltration of eosinophils in BAL fluid after CDE challenge, (ii) the serum level of rFel d 1-specific IgE was reduced and the level of IgG(2)a was more pronounced after CBP-rFel d 1 treatment, and (iii) there was also a tendency of decreased allergen-specific cellular response. CONCLUSIONS: Carbohydrate-based particles are effective tools as adjuvant and allergen carriers for use in ASIT and constitutes a promising strategy to improve allergy treatment.
机译：背景：过敏原特异性免疫疗法（ASIT）是唯一可以长期缓解症状的过敏性疾病。然而，对安全性和效率的关注突出了对治疗的改进的需要。我们以前曾提出基于碳水化合物的颗粒（CBP）作为ASIT的新型佐剂和过敏原载体。我们的这项研究的目的是使用对猫过敏的小鼠模型评估CBP在ASIT中的治疗潜力。方法：用重组（r）猫过敏原Fel d 1皮下免疫BALB / c小鼠，然后用猫皮屑提取物（CDE）鼻内攻击。用与CBP共价偶联的rFel d 1（CBP-rFel d 1）对致敏小鼠进行治疗。评估气道高反应性（AHR），支气管肺泡灌洗液（BAL）中白细胞的浸润，过敏原特异性血清免疫球蛋白水平和体外脾细胞反应。结果：CBP-rFel d 1处理的小鼠表现出过敏性炎症的特征减少。他们的反应是（i）CDE攻击后AHR明显降低，BAL液中的嗜酸性粒细胞浸润明显;（ii）CBP-后，血清rFel d 1-特异性IgE水平降低，IgG（2）a水平更加明显。 rFel d 1处理，以及（iii）也有降低变应原特异性细胞反应的趋势。结论：基于碳水化合物的颗粒是用于ASIT的佐剂和过敏原载体的有效工具，并且是改善过敏治疗的有前途的策略。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号