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首页> 外文期刊>Allergy >Galectin-10 mRNA is overexpressed in peripheral blood of aspirin-induced asthma.
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Galectin-10 mRNA is overexpressed in peripheral blood of aspirin-induced asthma.

机译：Galectin-10 mRNA在阿司匹林诱发的哮喘患者外周血中过表达。

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BACKGROUND: In sensitive patients, aspirin is associated with nasal and bronchial inflammation, eliciting local symptoms. Although the disease is clinically well characterized, its physiopathology is incompletely understood and noninvasive procedures, allowing an effective distinction between aspirin-induced asthma (AIA) and aspirin-tolerant asthma (ATA) are missing. OBJECTIVES: The aims of the study were to compare AIA and ATA cohorts for clinical characteristics and to screen peripheral blood for differential mRNA expression. METHODS: Patients experiencing symptoms following aspirin ingestion were considered as aspirin sensitive. Peripheral blood was collected to quantify mRNA expression, using microarray technology and quantitative RT-PCR. RESULTS: Data indicated that AIA and ATA share large number of similarities for clinical phenotype. Screening of mRNA expression using microarray showed an overexpression of galectin-10 mRNA in AIA (AIA/ATA ratio = 1.9, P < 0.05). Results were confirmed using qRT-PCR. A positive correlation was established between microarray and qRT-PCR results for galectin-10 mRNA expression (r = 0.92, P < 0.0001). Finally, qRT-PCR results were validated on a subset of asthmatics and controls, showing an increased expression of galectin-10 mRNA in AIA vs ATA (P < 0.001) and vs controls (P < 0.01). CONCLUSIONS: Our results demonstrate that AIA and ATA remain difficult to distinguish using clinical criteria. Employing two molecular biological methods, we demonstrate that galectin-10 mRNA is overexpressed in AIA, suggesting a novel candidate gene and a potentially innovative pathway for mucosal inflammation in aspirin intolerance.
机译：背景：在敏感的患者中，阿司匹林与鼻和支气管发炎有关，引起局部症状。尽管该疾病在临床上已被很好地表征，但其生理病理学尚未完全理解，并且无创性操作使得缺少阿司匹林诱导的哮喘（AIA）和阿司匹林耐受性哮喘（ATA）之间的有效区分。目的：本研究的目的是比较AIA和ATA队列的临床特征，并筛选外周血中的差异mRNA表达。方法：阿司匹林摄入后出现症状的患者被视为对阿司匹林敏感。使用微阵列技术和定量RT-PCR收集外周血以定量mRNA表达。结果：数据表明，AIA和ATA在临床表型上有很多相似之处。使用微阵列筛选mRNA表达显示在AIA中galectin-10 mRNA过表达（AIA / ATA比= 1.9，P <0.05）。使用qRT-PCR确认结果。 galectin-10 mRNA表达在芯片与qRT-PCR结果之间建立正相关（r = 0.92，P <0.0001）。最后，qRT-PCR结果在部分哮喘患者和对照组中得到验证，表明AIA与ATA（P <0.001）和对照组（P <0.01）的半乳凝素10 mRNA表达增加。结论：我们的结果表明，使用临床标准仍难以区分AIA和ATA。利用两种分子生物学方法，我们证明了半乳糖凝集素10 mRNA在AIA中过表达，表明阿司匹林耐受性中黏膜炎症的新候选基因和潜在创新途径。

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