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首页> 外文期刊>Allergy >Nasal CpG oligodeoxynucleotide administration induces a local inflammatory response in nonallergic individuals.
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Nasal CpG oligodeoxynucleotide administration induces a local inflammatory response in nonallergic individuals.

机译：鼻CpG寡脱氧核苷酸给药可在非过敏性个体中引起局部炎症反应。

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BACKGROUND: We have previously demonstrated the presence of toll-like receptor 9 in the nasal mucosa of both healthy and allergic individuals. CpG motifs, found in bacterial and viral DNA, elicit strong immunostimulatory effects via this receptor. CpG is known to skew the immune system towards a T helper 1 (Th1) profile, thereby suppressing Th2-driven allergic responses. This study was designed to examine the effects of CpG administration in the human nose. METHODS: Twenty subjects, of whom 10 suffered from seasonal allergic rhinitis (AR), were challenged intranasally with CpG outside pollen season. Symptom scores, nasal airway resistance (NAR), and nasal and pulmonary nitric oxide (NO) levels were assayed prior to challenge and 30 min, 6, 24 and 48 h post challenge. The presence of leukocytes and various cytokines were analyzed in nasal lavage (NAL) fluids before and after CpG exposure. RESULTS: Increased NAR, nasal NO production and secretion of interleukin (IL)-1beta, IL-6, and IL-8 were seen after CpG exposure. Further analysis revealed that this inflammatory response was more marked in healthy subjects than among patients with AR, although a higher basal inflammatory response was recorded in the allergic group. In vitro experiments suggest that the effects induced by CpG are mediated by epithelial cells and neutrophils. CONCLUSION: Nasal administration of CpG induces a local airway inflammation, more distinct among healthy than allergic individuals. The reduced responsiveness to CpG in allergic patients might be related to the ongoing minimal persistent inflammation. Results from cytokine analyses reflect the ability of CpG to induce a pro-inflammatory Th1-like immune response.
机译：背景：我们之前已经证明健康和过敏个体的鼻黏膜中存在Toll样受体9。在细菌和病毒DNA中发现的CpG基序通过该受体引起强烈的免疫刺激作用。已知CpG使免疫系统偏向T辅助1（Th1）谱，从而抑制Th2驱动的过敏反应。这项研究旨在检查CpG给药对人鼻的影响。方法：20名受试者，其中10名患有季节性过敏性鼻炎（AR），在花粉季节以外用CpG鼻内攻击。攻击前和攻击后30分钟，6、24和48小时分析症状评分，鼻气道阻力（NAR）以及鼻和肺一氧化氮（NO）水平。在暴露于CpG之前和之后，在鼻灌洗液中分析了白细胞和各种细胞因子的存在。结果：CpG暴露后，发现NAR，鼻腔NO的产生增加以及白介素（IL）-1beta，IL-6和IL-8的分泌增加。进一步的分析表明，健康人群中的这种炎症反应比AR患者更为明显，尽管在过敏组中基础炎症反应的发生率更高。体外实验表明，CpG诱导的作用是由上皮细胞和中性粒细胞介导的。结论：鼻内施用CpG会引起局部气道炎症，在健康人群中比在过敏人群中更为明显。变态反应患者对CpG的反应性降低可能与正在进行的轻微持续性炎症有关。细胞因子分析的结果反映了CpG诱导炎症性Th1样免疫应答的能力。

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